Abstract:

Sediment samples were collected from the northern Gulf of Mexico (29.3989N, -88.8678W) during an expedition with the R/V Weatherbird on August 21 in 2013, 87 kilometers NW from Macondo wellhead near the Mississippi river Delta at 56 m water depth. Surface (0-5 cm depth) sediment core samples were collected in sterile plastic bags for cultivation and immediately stored at 4˚C for 2 months until enrichment cultures were initiated. Sediment from the 0-5 cm depth interval was homogenized and supplemented as a 10% (w/v) inoculum with sterile anaerobic artificial seawater medium with either hexadecane or phenanthrene.

Suggested Citation:

Boryoung Shin, Karsten Zengler, Kuk-Jeong Chin, Will A Overholt, Joel E Kostka. 2018. Anaerobic degradation of hexadecane and phenanthrene coupled to sulfate reduction in seafloor sediments from the northern Gulf of Mexico. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7VD6X0R

Publications:

Shin, B., Kim, M., Zengler, K., Chin, K.-J., Overholt, W. A., Gieg, L. M., … Kostka, J. E. (2019). Anaerobic degradation of hexadecane and phenanthrene coupled to sulfate reduction by enriched consortia from northern Gulf of Mexico seafloor sediment. Scientific Reports, 9(1). doi:10.1038/s41598-018-36567-x

Purpose:

The objectives of this study were (i) to demonstrate hydrocarbon degradation coupled with sulfate reduction and (ii) to characterize the hydrocarbon-degrading microbial community in anoxic sediments of the northern GoM seafloor.

Data Parameters and Units:

Sediment samples collected from 56m water depth and 0-5cm surface sediment was used for experiment (Site name : SL1040) Experiment; Library name (name includes hydrocarbon used_DNA/RNA_replicate); MBases; MBytes; Run; SRA sample (SRS2928771); Assay type (AMPLICON); AvgSpotLen (502); BioProject (PRJNA433527); BioSample (SAMN08499993); BioSampleModel (MIMS.me); Consent (public); Insert size (0); Instrument (Illumina MiSeq); Library layout (PAIRED); Library selection (PCR); Library source (GENOMIC); Load date (MM/DD/YYYY); Organism (mixed culture metagenome); Platform (ILLUMINA); Release date (MM/DD/YYYY); SRA study (SRP132511); Sample name (Sulfate-reducing enrichment culture); Sulfate reducing enrichment (mixed culture); Collection date (MM/DD/YYYY); Depth (cm); Elevation (m); Environmental biome (sediment); Environmental material (sediment); Geographic location name (USA); Growth medium (artificial sea water media); Isolation source (Gulf of Mexico); Latitude Longitude (lat_lon, decimal degrees); Petroleum hydrocarbon (Phenanthrene or Hexadecane); Sample type (mixed culture); Carbon source (Phenanthrene and Hexadecane); Concentration (g/L); Sample type (DNA or RNA); Replicate; Duration (161 days of incubation after transfer)

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