Abstract:

Lipid class composition by Iatroscan of adult Florida pompano exposed to south Louisiana crude oil via CEWAF-spiked water.

Suggested Citation:

DL Wetzel, TA Sherwood, CA Miller, RL Medvecky. 2019. CEWAF water exposed Florida pompano F0 reproduction: Lipid Class Composition. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N77S7M7R

Purpose:

Controlled oil exposure study to evaluate the effects of crude oil on lipid structure in whole body of fish.

Data Parameters and Units:

T208 Sample Key worksheet: Collected date (MM/DD/YYYY), Field ID, Treatment, Spawning Tank, Matrix, MML Number, Species, Gender, Weight (g), Length (cm). Tank Descriptions worksheet: Tank, # Female, # Male, Treatment, Start and End Date (MM/DD/YYYY), Date Spike A (MM/DD/YYYY), Time Spike A, Date Spike B (MM/DD/YYYY), Time Spike B. CEWAF Dilution Preparation worksheet: Date (MM/DD/YYYY), Time, Carboy ID, Stock Vol (L), Final Vol (L). % Lipid worksheet: Treatment, Fish, MML Liver, Liver % Lipid, MML Whole Body, Whole Body % Lipid. Liver TLC Results worksheet: Treatment, Fish ID, Rep, Liver MML #, % Wax esters and Sterol esters, % Triglycerides, % Free fatty acids, % Cholesterol, % Phospholipids and Polar lipids. Whole Body TLC Results worksheet: Treatment, Fish ID, Rep, Whole Fish MML #, % Wax esters and sterol esters, % Triglycerides, % Free fatty acids, % Cholesterol, % Phospholipids and Polar lipids.

Methods:

Tissue samples were extracted via EPA method 3545 using a Dionex ASE300, then concentrated to 10 mL in DCM for analysis. Lipid Composition: 1 uL of sample analyzed with Iatroscan using PeakSimple 444 software.

Instruments:

Iatroscan MK-6s

Error Analysis:

QA measures were included into per batch according to Environmental Laboratory for Forensics Quality Assurance Plan. If QA measurements were not met, samples were re-analyzed or data was qualified.

Individual Files: