Abstract:

Controlled oil exposure study to evaluate the effects of south Louisiana crude oil, via contaminated sediment, on fish health. Cytokine concentrations were analyzed in southern flounder after exposure to oil-contaminated sediment for 30 days. This dataset contains Ct and percent efficiencies of deferentially expressed genes of southern flounder exposed to sediments contaminated with south Louisiana crude oil.

Suggested Citation:

Dana Wetzel. 2019. Sub-adult southern flounder exposure to crude oil-contaminated sediment: Immune Function Assays & Gene Expression. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-rhvn-dx52

Publications:

Sherwood, T. A., Medvecky, R. L., Miller, C. A., Tarnecki, A. M., Schloesser, R. W., Main, K. L., … Wetzel, D. L. (2019). Nonlethal Biomarkers of Oxidative Stress in Oiled Sediment Exposed Southern Flounder (Paralichthys lethostigma): Utility for Field-Base Monitoring Exposure and Potential Recovery. Environmental Science & Technology, 53(24), 14734–14743. doi:10.1021/acs.est.9b05930

Purpose:

Controlled oil exposure study to evaluate the effects of south Louisiana crude oil, via contaminated sediment, on fish health.

Data Parameters and Units:

Test 211 Sample Key worksheet: Collected date (MM/DD/YYYY), Field ID, Matrix, MML Number, Species, Gender, Weight (g), Length (cm), Treatment. Tank Description worksheet: Tank ID, Sediment Start date (MM/DD/YYYY), Fish Added date, Treatment, Number of fish. Sediment Preparation worksheet: Date, Time, Tank, Oil (g), Oil ID, Sediment (kg), Prepared by. ELISA worksheet: Treatment, Fish ID, MML, dilution factor, Cusabio TNFa (pg/mL) Rep1 (pg/mL), Rep2 (pg/mL), avg (pg/mL), avg-PBS (pg/mL). Gill gene expression worksheet: Matrix, Rep, MML, Treatment, Ct of genes: EF-1a House Keeping Gene, IL8, IL1B, IL6, TNF-a, IgM, IFN-g. BDL=below detection limit. Gill efficiencies worksheet: Matrix, Rep, MML, Treatment, EF-1a House Keeping Gene, IL8, IL1B, IL6, TNF-a, IgM, IFN-g. BDL=below detection limit. Liver gene expression worksheet: Matrix, Rep, MML, Treatment, Ct of genes: EF-1a House Keeping Gene, IL8, IL1B, IL6, TNF-a, IgM, GST, sfCYP1a, ofCYP1a, IFN-g. NA=samples analyzed, but no data available (i.e. below detectable limit). Efficiencies liver worksheet: Matrix, Rep, MML, Treatment, EF-1a House Keeping Gene, IL8, IL1B, IL6, TNF-a, IgM, GST, sfCYP1a, ofCYP1a, IFN-g. NA=samples analyzed, but no data available (i.e. below detectable limit).

Individual Files: