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Red drum CEWAF uptake/depuration study: Immune Function Assays & Gene Expression

Authors: Dana Wetzel
Published On: Feb 11 2019 21:30 UTC
DOI: https://doi.org/10.7266/n7-5hb7-zg41
UDI: R4.x267.000:0065
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Project Information

Funded By:
Gulf of Mexico Research Initiative

Funding Cycle:
RFP-IV

Research Group:
Center for the Integrated Modeling and Analysis of Gulf Ecosystems II (C-IMAGE II)

Point of Contact

Dana Wetzel
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
dana@mote.org

Theme keywords

red drum, cytokines, gene expression, Sciaenops ocellatus

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Abstract:

Cytokine concentrations in red drum after exposure to chemically enhanced water accommodated fraction (CEWAF) of south Louisiana crude oil. Ct and percent efficiencies of the deferentially expressed genes of red drum spleen, liver and gill tissue exposed to south Louisiana crude oil CEWAF are provided.

Suggested Citation:

Dana Wetzel. 2019. Red drum CEWAF uptake/depuration study: Immune Function Assays & Gene Expression. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-5hb7-zg41

Purpose:

To determine the effects of CEWAF exposure on red drum.

Data Parameters and Units:

Tank Descriptions worksheet: Tank ID, Start and End Date (MM/DD/YYYY), Treatment, Number of Fish, Notes, Recorded by. CEWAF Prep worksheet: Date (MM/DD/YYYY), Carboy, Oil (g), Corexit (g), Volume Water (L). Test 113 Sample Key worksheet: Day, Sample Date (MM/DD/YYYY), Sample Location (MAP - Mote Aquaculture Park), Tank ID (numbers in fron of Tank indicate tagged fish), Matrix, MML #, Fish ID, Treatment. ELISA worksheet: Whole Body MML, Whole Body tPAH (ng/g), Liver MML, Liver tPAH (ng/g), Fish ID, Day, Tank, Treatment. MML, rep 1, rep2, avg, stdev, %cv for lgM, IL-1B Abbexa, IL-8 MBS (for two dates), TNFa MBS (for two dates), IL-IB MBS (for two dates), MBS CXCL10. Units in pg/ml. Spleen ct worksheet: Matrix, Rep, MML, Treatment, Day, BA (Ct) House keeping gene, CXC10, fucolectin, GST (0.9), GST (1.5), CYP1A, UDP60, CYP26C1, GS. spleen efficiencies worksheet: Matrix, Rep, MML, Treatment, Day, BA (Ct) House keeping gene, CXC10, fucolectin, GST (0.9), GST (1.5), CYP1A, UDP60, CYP26C1, GS. Liver Ct worksheet: Matrix, Rep, MML, Treatment, Day, BA (Ct) House keeping gene, CXC10, CCL20, C4, fucolectin, GST (0.9), GST (1.5), CYP1A, UDP60, CYP26C1, GS. Liver efficiencies worksheet: Matrix, Rep, MML, Treatment, Day, BA (Ct) House keeping gene, CXC10, CCL20, C4, fucolectin, GST (0.9), GST (1.5), CYP1A, UDP60, CYP26C1, GS. Gill Ct worksheet: Matrix, Rep, MML, Treatment, Day, BA (Ct) House keeping gene, fucolectin, GST (0.9), CYP1A. Gill efficiencies worksheet: Matrix, Rep, MML, Treatment, Day, BA (Ct) House keeping gene, fucolectin, GST (0.9), CYP1A.

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