Abstract:

This dataset contains results of lipid class composition using Iatroscan and fatty acid identification of Florida pompano exposed to dispersed south Louisiana crude oil. Florida pompano were either exposed to 1 or 0.5 ppm of chemically enhanced water accommodated fraction (CEWAF). A control experiment was also performed. After a specified period fish were dissected and lipids were extracted from liver tissue and whole body tissue and were analyzed.

Suggested Citation:

Dana Wetzel. 2019. CEWAF-exposed Florida pompano reproduction: Lipid Class Composition. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-m5b4-9956

Purpose:

This is part of an experiment to see the effects of dispersed crude oil, via south Louisiana crude oil CEWAF, on the lipid content of fish.

Data Parameters and Units:

Sample key worksheet: MML, Matrix (Liver, Whole Body), Tag ID, Collected date (dd-mon-yy), Organism Species (Florida Pompano), Treatment (Control, Low, High), Spawning Tank. Exposure tank description and spawning tank description worksheets: Tank ID (A, B, C, D, E, F, G, H, I), Start date (mm/dd/yyyy), End date (mm/dd/yyyy), Treatment (High CEWAF, Low CEWAF, Control), Number of Fish (5), Notes, Recorded by (RM). CEWAF Prep worksheet: Start Date (mm/dd/yyyy), End Date (mm/dd/yyyy), Carboy (A, B, F), Oil (g), Corexit (g), Volume Water (L), Treatment (High, Low), Amount of Stock Solution (L), Amount of Seawater (L). % Lipid worksheet: Dissection Date (dd-mon-yy), Exposure Tank ID (Control, Low, High), Tag number, Liver, Lipid (%), Whole Body, Lipid (%). Liver TLC results and whole body TLC results worksheets: Treatment (Control, Low, High), Tag number, Rep, MML, Wax esters & Sterol Esters (%), Triglycerides (%), Free Fatty Acids (%), Cholesterol (%), Phospholipids & Polar Lipids (%).

Methods:

Methods adapted from the NOAA technical memorandum Sloan et al., 2014.

Instruments:

Mitsubishi Kagaku Iatron, Inc., Iatroscan MK‌-6s, PeakSimple Chromatography Data System.

Provenance and Historical References:

Sloan, C. A., B. F. Anulacion, K. A. Baugh, J. L. Bolton, D. Boyd, R. H. Boyer, D. G. Burrows, D. P. Herman, R. W. Pearce, G. M. Ylitalo. (NWFSC) 2014. Northwest Fisheries Science Center's analyses of tissue, sediment, and water samples for organic contaminants by gas chromatography/mass spectrometry and analyses of tissue for lipid classes by thin layer chromatography/flame ionization detection. U.S. Dept. of Commerce, NOAA Tech. Memo., NMFS-NWFSC-125, 61 p.

Individual Files: