Dataset Available

Red drum diet exposure to Deepwater Horizon crude oil contaminated feed: Gene Expression

Authors: Dana Wetzel
Published On: Feb 11 2019 21:06 UTC
DOI: https://doi.org/10.7266/n7-7abz-hq39
UDI: R4.x267.000:0056
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No. of Files: 1

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Project Information

Funded By:
Gulf of Mexico Research Initiative

Funding Cycle:
RFP-IV

Research Group:
Center for the Integrated Modeling and Analysis of Gulf Ecosystems II (C-IMAGE II)

Point of Contact

Dana Wetzel
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
dana@mote.org

Theme keywords

red drum, gene expression, spleen, liver, Sciaenops ocellatus

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Abstract:

This dataset contains Ct values and efficiencies of deferentially expressed genes of red drum spleen and liver tissues after exposure via pellets saturated with south Louisiana crude oil for 14 days.

Suggested Citation:

Dana Wetzel. 2019. Red drum diet exposure to Deepwater Horizon crude oil contaminated feed: Gene Expression. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-7abz-hq39

Purpose:

To examine deferentially expressed genes of red drum spleen and liver tissues after exposure via pellets saturated with south Louisiana crude oil for 14 days.

Data Parameters and Units:

Test 103 Sample Key worksheet: Date Collected (MM/DD/YYYY), Location Collected: MAP (Mote Aquaculture Park), Field ID, Matrix, MML, Species, Tank ID. Food Preparation worksheet: Prep start and end date (MM/DD/YYYY), Est. total mass needed for daily feeding and samples (g), Feed Prep. Method, Feed mass (g), Mass of oil added (g), Mixing Speed, Mixing Time (minutes), Oil Sources, Recorded by. spleen Ct worksheet: Matrix, Tank, MML, Treatment, BA (ct) House Keeping Gene, fuco, cyp1a, gst_0.9, CCL20, C4, CXCL10, UDP60, GS, cyp26c1. spleen Efficiencies worksheet: Matrix, Tank, MML, Treatment, BA (ct) House Keeping Gene, fuco, cyp1a, gst_0.9, CCL20, C4, CXCL10, UDP60, GS, cyp26c1. Liver Ct worksheet: Matrix, Tank, MML, Treatment, BA (ct) House Keeping Gene, fuco, cyp1a, gst_0.9, gst_1.5, cxcl10.

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