GRIIDC | R4.x267.000:0054

Dataset Available

Red drum diet exposure to Deepwater Horizon crude oil contaminated feed: Lipid Composition

Authors: DL Wetzel, TA Sherwood, CA Miller, RL Medvecky

Published On: Dec 23 2018 22:23 UTC

DOI: https://doi.org/10.7266/N7TT4PFR

UDI: R4.x267.000:0054

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Project Information

Funded By:
Gulf of Mexico Research Initiative

Funding Cycle:
RFP-IV

Research Group:
Center for the Integrated Modeling and Analysis of Gulf Ecosystems II (C-IMAGE II)

Point of Contact

Dana Wetzel
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
dana@mote.org

Theme keywords

Diet exposure, Deepwater Horizon, crude oil, lipid, phospholipid, red drum, Sciaenops ocellatus

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Abstract:

Lipid class composition of red drum fed a diet of pellets saturated with south Louisiana crude oil using Iatroscan.

Suggested Citation:

DL Wetzel, TA Sherwood, CA Miller, RL Medvecky. 2018. Red drum diet exposure to Deepwater Horizon crude oil contaminated feed: Lipid Composition. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7TT4PFR

Purpose:

Controlled oil exposure study to evaluate the effects of crude oil on lipid structure in whole body of fish.

Data Parameters and Units:

Test 103 Sample Key worksheet: Date Collected (MM/DD/YYYY), Location Collected (MAP - Mote Aquaculture Park), Field ID, Matrix, MML, Species, Project, Tank ID, Treatment (High, Low, Control). Food Preparation worksheet: Prep start and end date, est. total mass needed for daily feeding and samples (g), feed prep. method, feed mass (g), mass of oil added (g), mixing speed, mixing time, oil source. % Lipid worksheet: FISH ID, Tank ID, Whole Body (sample ID), % Lipid, Liver (sample ID), % Lipid. Liver TLC Results worksheet: Fish ID, Tank ID, REP (replicate), Liver MML #, % Wax esters & Sterol Esters, % Triglycerides, % Free Fatty Acids, % Cholesterol, % Phospholipids & Polar Lipids. Whole Body TLC Results worksheet: Fish ID, Tank ID, REP (replicate), Liver MML #, % Wax esters & Sterol Esters, % Triglycerides, % Free Fatty Acids, % Cholesterol, % Phospholipids & Polar Lipids.

Methods:

Tissue samples extracted via EPA method 3545 using a Dionex ASE300, then concentrated to 10 mL in DCM for analysis. Lipid Composition: 1 uL of sample analyzed with Iatroscan using PeakSimple 444 software.

Instruments:

Iatroscan MK-6s

Error Analysis:

QA measures were included into per batch according to Environmental Laboratory for Forensics Quality Assurance Plan. If QA measurements were not met, samples were re-analyzed or data was qualified.