Abstract:

This dataset contains concentrations and ratios of oil compounds (e.g., n-alkanes, isoprenoids, polycyclic aromatic hydrocarbons (PAHs), hopanes, and steranes) analyzed from crude oil biodegradation experiments using shallow and deep northern Gulf of Mexico sediments.

Suggested Citation:

Sara Lincoln, Katherine Freeman. 2017. Organic geochemical analysis of crude oil biodegradation experiments using northern Gulf of Mexico sediments collected from sites at 60m to 1500m water depth. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7833QFK

Publications:

Nguyen, U. T., Lincoln, S. A., Valladares Juárez, A. G., Schedler, M., Macalady, J. L., Müller, R., & Freeman, K. H. (2018). The influence of pressure on crude oil biodegradation in shallow and deep Gulf of Mexico sediments. PLOS ONE, 13(7), e0199784. doi:10.1371/journal.pone.0199784

Purpose:

To study the influence of pressure on rates and patterns of crude oil biodegradation.

Data Parameters and Units:

Sediment #, Sample number, Reactor/dummy, Cruise, Site, Horizon (mm), Water depth = depth that sediment was collected (m), Latitude = latitude where sediment was collected (decimal degrees), Longitude = longitude where sediment was collected (decimal degrees), CTD bottom water T = temperature at bottom of water column measured by CTD (deg C), CTD bottom water O2 = oxygen concentration at bottom of water column measured by CTD (umol/kg), Incubation temperature (Cooling Chamber at 4°C, I = Incubator at 10°C, RT - Room Temperature), P initial = initial pressure in reactor measured (bar), P final = final pressure in reactor (bar), Total organic carbon (TOC, weight %), Carbonate content (weight %), concentrations of : C13 to C40 n-alkane, pristane , C2-C4 naphthalene , C1-C3 fluorene phenanthrene - C4-phenanthrene, dibenzothiophene-C3-diobenzothiophene, C1-C3 pyrene, 11H-benzoF, chrysene - C3 chrysene in nanograms in sediment and water for each sample; peak area of C30-hopane and C26-triaromatic sterane; peak high of alkylated PAHs GCMS peak area

Methods:

Experiment was run from 10/10/2014-10/28/2014 Laboratory incubation of sediments with crude oil Extraction of sediments with 9:1 Dichloromethane: Methanol Silica gel column chromatography Gas chromatography – mass spectrometry Acid digestion Leco Carbon/Sulfur analyzer

Instruments:

GCMS, Leco Carbon/Sulfur Analyzer

Error Analysis:

GCMS: We report % variability for internal and external standards run daily during analysis

Individual Files: