Abstract:

Lionfish were captured from various locations in the northern Gulf of Mexico from natural and artificial reefs during 2013-03-30 to 2014-03-08. Diet data were collected and analyzed using visual identification and DNA barcoding analyses. This dataset supports the publication: Dahl, K.A., Patterson, W.F., Robertson, A.E., & Ortmann, A.C. (2017). DNA barcoding significantly improves resolution of invasive lionfish diet in the Northern Gulf of Mexico. Biological Invasions, 19, 1917-1933. doi:10.1007/s10530-017-1407-3

Suggested Citation:

Kristen Dahl, William Patterson. 2018. DNA barcoding of invasive lionfish diet in the northern Gulf of Mexico, 2013-03-30 to 2014-03-08. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7F769ZD

Publications:

Dahl, K. A., Patterson, W. F., Robertson, A., & Ortmann, A. C. (2017). DNA barcoding significantly improves resolution of invasive lionfish diet in the Northern Gulf of Mexico. Biological Invasions, 19(6), 1917–1933. doi:10.1007/s10530-017-1407-3

Dahl, K. A., Portnoy, D. S., Hogan, J. D., Johnson, J. E., Gold, J. R., & Patterson, W. F. (2018). Genotyping confirms significant cannibalism in northern Gulf of Mexico invasive red lionfish, Pterois volitans. Biological Invasions, 20(12), 3513–3526. doi:10.1007/s10530-018-1791-3

Purpose:

Observe trends in diet of invasive red lionfish (Pterois volitans) in the northern Gulf of Mexico using DNA barcoding.

Data Parameters and Units:

Site Details worksheet: Site (site location name), Lat (latitude in degrees decimal minutes), Long (longitude in degrees decimal minutes), Habitat (habitat type: AR [artificial reef], NR [natural reef]). Lionfish Sample Data worksheet: Field ID (unique sample number), Date of Capture (date of sample collection), Site (site location name), Species (fish species sampled), Habitat (habitat type: AR [artificial reef], NR [natural reef]), Size Class (size categories by total length: S=small [<200mm TL], M=medium [200-250mm TL], L=large [>250mm TL]), Season (sample timing by season: S=spring, U=summer, F=fall, W=winter), TL (total length, mm), SL (standard length, mm), Mass (kg), Sex (sex of lionfish), Gape Width (mm, lionfish), Gape Height (mm, lionfish), Stomach Mass (weight of stomach, g). Diet Data - Artificial Reefs and Natural Reefs worksheets: Field ID (unique sample number), Prey Taxa (lowest level identification of prey), Prey Family/Order (higher order identification of prey), Prey Category (functional groups of prey), Dry Mass (dry mass of prey, g). Barcode ID (unique sample number for barcoded prey), Field ID (unique sample number), Within Field ID (prey identifier within each individual field sample), Simulated Dry Mass (dry mass simulated from digestion stage and wet/dry conversion factor), Consensus BP (length of prey COI barcoding sequence), Accession Number (unique GenBank identifier of voucher COI sequences), Best Match (GenBank or BOLD closest taxa identification from BLAST search), Taxon (level of identification based on sequence quality and similarity of voucher sequences), Taxon ID (final identification of prey made from DNA barcoding), Common Name (common name of barcoded prey), E-value (measure of sequence match: the number of GenBank search hits with at least this score that you would expect purely by chance), % Identical (% similarity between barcode and voucher sequences), % Grade (measure of sequence match: percentage calculated from query coverage, E-value and % identical), NCBI Accession Number.

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