Abstract:
This dataset contains values for potential rates of nitrification, abundances of ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA), denitrifying bacteria (nirS), methane-oxidizing bacteria (MOB) and soil properties collected from 12 marshes in Terrebonne Bay (TB), western Barataria Bay (WB), and eastern Barataria Bay (EB), Louisiana during July sampling campaigns in 2015 and 2016. In each region, we sampled two pairs of oiled and unoiled marshes. At each site, we collected 5-cm deep soil cores at 4 locations (5, 10, 15, and 20m from the marsh edge). Each sample was analyzed for potential nitrification rates with AOA, AOB, nirS, and MOB abundances also quantified in July of each year. Additionally, we measured a series of basic site characterizations: soil properties, soil extractable nutrients, porewater, overlying water and adjacent bay water depth, temperature, salinity, specific conductance and nutrients, when available.
Suggested Citation:
Roberts, Brian J.. 2019. Marsh soil nitrification rates, microbial communities, and explanatory variables collected from marshes in Terrebonne Bay, western Barataria Bay, and eastern Barataria Bay, Louisiana from July 2015 to July 2016. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-0svp-nn22
Purpose:
This dataset was developed as part of a research project investigating the effects of the Deepwater Horizon oil spill on salt marsh biogeochemistry. This component of the CWC research effort addressed the following questions: 1) Do nitrification potential rates spatially across the LA coast and/or with marsh position?; 2)What are the primary drivers of nitrification rates?; 3) Are these results different between oiled and unoiled marshes?; and 4) Is there interannual variability in any of these patterns or responses to oiling? This dataset is a continuation of study begun with dataset R1.x139.143-0001 (2012) and dataset R1.x139.143-0032 (2013-2014).
Data Parameters and Units:
Region-site ID: User-defined identifier assigned to each sampling site. Identifier contains a region code (TB=Terrebonne Bay, WB= western Barataria Bay, EB=eastern Barataria Bay) and a site number designation; Region: Region of sample collection (TB=Terrebonne Bay, WB= western Barataria Bay, EB=eastern Barataria Bay); Site: Site number designation; Latitude (decimal degrees); Longitude (decimal degrees); Oil status: Indicates whether the marsh received Macondo Oil (Oiled) or not (Unoiled); Plot: Plot identifier along the sampling transect; Distance (m): Distance from the marsh edge of the sampling plot (meters); Month-Year: Indicates the month and year that samples were collected or measurements made; Nitrification potential (nmol NO3-N gdw-1 d-1): rate of nitrification potential in the top 5 cm of soil at the plot; AOA (copies/gdw): abundance of ammonia-oxidizing archaea in top 5 cm of soil at the plot, expressed in amoA gene copies gdw-1; AOB (copies/gdw): abundance of ammonia-oxidizing bacteria in top 5 cm of soil at the plot, expressed in amoA gene copies gdw-1; nirS (copies/gdw): abundance of denitrifying bacteria in top 5 cm of soil at the plot, expressed in nirS gene copies gdw-1; MOB (copies/gdw): abundance of methane-oxidizing bacteria in top 5 cm of soil at the plot, expressed in pmoA gene copies gdw-1; Soil T (°C): soil temperature at the plot, in degrees C; soil pH: pH of the top 5 cm of soil at the plot; soil Eh: soil redox potential (Eh) in the surface soil of the plot (units = mV); soil bulk density (g/cm3): bulk density of the top 5 cm of soil at the plot; soil organic C (%): organic carbon content of the top 5 cm of soil at the plot; soil total N (%): total nitrogen content of the top 5 cm of soil at the plot; soil total P (µg/g): total phosphorus content of the top 5 cm of soil at the plot; soil CN (mol:mol): organic carbon to total nitrogen ratio of the top 5 cm of soil at the plot; soil NP (mol:mol): total nitrogen to total phosphorus ratio of the top 5 cm of soil at the plot; soil CP (mol:mol): organic carbon to total phosphorus ratio of the top 5 cm of soil at the plot; soil water content (%): gravimetric water content of the top 5 cm of soil at the plot; soil extractable NO3 (micromols per grams dry weight of soil): extractable nitrate + nitrite concentration of the top 5 cm of soil at the plot; soil extractable NH4 (micromols per grams dry weight of soil): extractable ammonium concentration of the top 5 cm of soil at the plot; soil extractable PO4 (micromols per grams dry weight of soil): extractable orthophosphate concentration of the top 5 cm of soil at the plot; Porewater salinity (psu): salinity of porewater from the plot Overlying; water depth (cm): depth of overlying water at plot; Overlying water temp (°C): temperature of overlying water at plot; Overlying water salinity (psu): salinity of overlying water at plot; Overlying water specific conductance (mS): specific conductance of overlying water at plot; Baywater temp (°C): temperature of baywater adjacent to site; Baywater salinity (psu): salinity of baywater adjacent to site; Baywater specific conductance (mS): specific conductance of baywater adjacent to site; Baywater NO3-N + NO2-N (micromols per liter): dissolved nitrate + nitrite concentration of baywater adjacent to site; Baywater NH4-N (micromols per liter): dissolved ammonium concentration of baywater adjacent to site; Baywater PO4-P (micromols per liter): dissolved orthophosphate concentration of baywater adjacent to site; Baywater SiO2 (micromols per liter): dissolved silicate concentration of baywater adjacent to site.
Provenance and Historical References:
Marton, J. M., Roberts, B. J., Bernhard, A. E., & Giblin, A. E. (2015). Spatial and Temporal Variability of Nitrification Potential and Ammonia-Oxidizer Abundances in Louisiana Salt Marshes. Estuaries and Coasts. doi:10.1007/s12237-015-9943-5
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