Abstract:

This dataset contains values for abundances of ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA), methane-oxdizing bacteria (MOB), and denitrifying bacteria (DNB) collected from 12 marshes in Terrebonne Bay (TB), western Barataria Bay (WB), and eastern Barataria Bay (EB), LA. In each region, we sampled two oiled marshes and two unoiled marshes. At each marsh, we collected 5-cm deep soil cores along a 20-m transect from the marsh edge and collecting soil samples every 5 m. Each sample was analyzed by quantitative PCR for abundance of AOB (betaproteobacterial amoA genes), AOA (archaeal amoA genes), MOB (pmoA genes), and DNB (nirS genes). Samples were collected in July 2015 and July 2016.

Suggested Citation:

Bernhard, Anne E.. 2019. Bacterial and archaeal gene abundances in Barataria and Terrebonne Bays in 2015-2016. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7319SXQ

Publications:

Bernhard, A. E., Chelsky, A., Giblin, A. E., & Roberts, B. J. (2019). Influence of Local and Regional Drivers on Spatial and Temporal Variation of Ammonia‐Oxidizing Communities in Gulf of Mexico Salt Marshes. Environmental Microbiology Reports. doi:10.1111/1758-2229.12802

Purpose:

This dataset is to complete a 5-year longitudinal study (started in 2012) on the microbial communities involved in nitrogen-cycling in the marshes, and to continue to monitor these marshes for impacts from the oil spill.

Data Parameters and Units:

Site name: identifies each site (there are 4 sites within each region) Year: the year the samples were collected Month: the month the samples were collected Region: identifies the region (TB for Terrebonne Bay, WB for western Barataria Bay, PS for eastern Barataria Bay/Port Sulphur) Position/Plot: each site has four plots at increasing distance from marsh edge (5m, 10m, 15m, 20m) oil status: oiled indicates sites that received Macondo oil AOA, gene copies g dry wt sediment-1: number of copies of the archaeal ammonia monooxygenase genes (amoA) AOB, gene copies g dry wt sediment-1: number of copies of the betaproteobacterial ammonia monooxygenase genes (amoA) MOB, gene copies g dry wt sediment-1: number of copies of the bacterial methane monooxygenase genes (pmoA) DNB, gene copies g dry wt sediment-1: number of copies of the dissimilatory nitrite reductase genes (nirS) Site Latitude (decimal degrees) Longitude (decimal degrees) TB1 29.29297, -90.604939 TB2 29.2915, -90.602824 TB3 29.304486, -90.565818 TB4 29.2964, -90.549793 WB1 29.288949, -90.042667 WB2 29.265837, -90.024864 WB3 29.2801, -89.975833 WB4 29.276533, -89.97535 PS3A 29.4608, -89.8750 PS5 29.4508, -89.7500 PS6 29.4458, -89.8761 PS7 29.4742, -89.8564

Methods:

Gene abundances were measured by quantitative PCR using the following primers: amoA1F/amoA2R for AOB, AamoA26F/AamoA417R for AOA, mb661 and A189 for MOB, nirS1F and nirS3R for DNB

Instruments:

QPCRs were run on a BioRad iCycler

Individual Files: