Dataset Available

Characterization of Chandeleur Island, Louisiana, Salt Marsh Microbial Communities: A 6 year post-Deepwater Horizon Impact Study, May 2016-February 2017

Authors: Sobecky, Patricia; Flournoy, Nikaela
Published On: May 07 2018 17:05 UTC
DOI: https://doi.org/10.7266/N7V69H36
UDI: R4.x262.000:0036
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Project Information

Funded By:
Gulf of Mexico Research Initiative

Funding Cycle:
RFP-IV

Research Group:
Alabama Center for Ecological Resilience (ACER)

Point of Contact

Patricia Sobecky
The University of Alabama / Department of Biological Sciences
psobecky@ua.edu

Data Collection Period
2016-05-24
to
2017-02-23
Theme keywords

denitrification, biodiversity, 16S rRNA amplicon sequencing, salt marsh, Deepwater Horizon

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Abstract:

As oil was deposited differentially within Chandeleur Island salt marshes, it was important to investigate the microbiology of oil-impacted areas as marsh vegetation is directly linked to microbe-driven ecosystem services such as denitrification, a nitrogen (N) cycle pathway. Genomic DNA was extracted from the top 0-7cm of sediment cores at six time points (May 2016, June 2016, September 2016, October 2016, November 2016, February 2017) from three salt marsh locales within the Chandeleur Islands. Microbial community abundance and biodiversity were characterized with 16S rRNA using Illumina MiSeq 2x 300 bp technology for 17 samples.

Suggested Citation:

Sobecky, Patricia; Flournoy, Nikaela. 2018. Characterization of Chandeleur Island, Louisiana, Salt Marsh Microbial Communities: A 6 year post-Deepwater Horizon Impact Study, May 2016-February 2017. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7V69H36

Publications:

Tatariw, C., Flournoy, N., Kleinhuizen, A. A., Tollette, D., Overton, E. B., Sobecky, P. A., & Mortazavi, B. (2018). Salt marsh denitrification is impacted by oiling intensity six years after the Deepwater Horizon oil spill. Environmental Pollution, 243, 1606–1614. doi:10.1016/j.envpol.2018.09.034

Purpose:

This 16S rRNA amplicon dataset is a 6 year post-Deepwater Horizon impact study which aimed to characterize the microbial biodiversity of three salt marsh locales within the Chandeleur Islands.

Data Parameters and Units:

Sequence data has been submitted to NCBI. To browse data, use SRA RunSelector: https://www.ncbi.nlm.nih.gov/Traces/study/?acc=SRP133272 To see summary list view: https://www.ncbi.nlm.nih.gov/sra/?term=SRP133272 The accessions: STUDY: PRJNA433990 (SRP133272) The salt_marsh_microbial_communities.csv file contains detail information regarding the submission at NCBI: BioSample, Experiment, Library Name, MBases, MBytes, Run, SRA Sample, Sample Name, Collection date (DD-MMM-YY), lat lon (latitude longitude, decimal degrees), Assay Type, AvgSpotLen, BioProject, BioSampleModel, Consent, InsertSize, Instrument, LibraryLayout, LibrarySelection, LibrarySource, Organism, Platform, SRA_Study, geo loc name (USA: Chandeleur Islands), host, isolation source (cm)

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