Abstract:

The effect of extracellular polymeric substances (EPS) extracted from Sagittula stellata and natural Gulf of Mexico bacterial consortia on aggregation of 1 micron latex particles on a crude oil drop surface is observed. Crude oil droplets between 100-200 microns are pinned in a microchannel while time lapse microscopy observes particle aggregation with time. Aggregation rates correlate positively with increasing protein-carbohydrate ratios in the varying EPS compositions studied.

Suggested Citation:

Andrew White, Jian Sheng. 2018. Aggregation of 1 micron latex spheres suspended in sterile EPS isolated from bacterial isolates and consortia on a micro-scale crude oil droplet. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7BV7F6V

Publications:

White, A. R., Jalali, M., Boufadel, M. C., & Sheng, J. (2020). Bacteria forming drag-increasing streamers on a drop implicates complementary fates of rising deep-sea oil droplets. Scientific Reports, 10(1). doi:10.1038/s41598-020-61214-9

White, A. R., Jalali, M., & Sheng, J. (2020). Hydrodynamics of a Rising Oil Droplet With Bacterial Extracellular Polymeric Substance (EPS) Streamers Using a Microfluidic Microcosm. Frontiers in Marine Science, 7. doi:10.3389/fmars.2020.00294

Purpose:

To investigate the effects of EPS composition on aggregation on a crude oil droplet.

Data Parameters and Units:

Microscope images (.tif), crude oil drop diameter (microns), protein concentration (mg/L), flow velocity (mm/s) The folders with_beads1" and "with_beads2" in the Sagittula_nonattached folder refer to two independent experiments with identical treatment of 1um latex beads. Beads concentration is 10^8 or 100million beads per mil.

Individual Files: