Abstract:

This dataset is comprised of data that can be used to determine resting metabolic rate and response to hypoxia in in four populations of Gulf killifish (Fundulus grandis) embryos. Two of these populations have been identified to be resistant to cardiac teratogenicity (Vince Bayou - VB and Paul bayou - PB) associated with exposure to polyaromatic hydrocarbons (PAHs) Two reference populations (Gang's bayou - GB and Smith Point - SP)were utilized for comparison. All data is in spreadsheet format. Declining oxygen, as organisms consumed oxygen, are reported for each population in millimoles oxygen in each 3 ml chamber. Mass and times of measurement are reported for calculation of metabolic rate as the rate of oxygen consumption per mass of each animal per unit time. Response to hypoxia is reported as time until larvae lost equilibrium during exposure to severe hypoxia. Response to hypoxia can be calculated as the time until loss of equilibrium per mass of the animal. Mass of larvae is reported to standardize for size differences in size.

Suggested Citation:

Benjamin Dubansky. 2016. Oxygen consumption and response to hypoxia in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds (DLCs). Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N78S4MWF

Publications:

Oziolor, E. M., Dubansky, B., Burggren, W. W., & Matson, C. W. (2016). Cross-resistance in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds. Aquatic Toxicology, 175, 222–231. doi:10.1016/j.aquatox.2016.03.019

Purpose:

This dataset was produced to determine metabolic rates of larval fish from different populations and to determine the response to acute low oxygen (hypoxia).

Data Parameters and Units:

Plate ID; Well; Population (Pop, site abbreviation); Oxygen content (millimoles); Mass (WM (wet mass), DM (dry mass), grams or milligrams); Time (seconds and minutes); Loss of equilibrium (LOE) Sampling site (abbreviation) latitude (decimal degrees) longitude (decimal degrees) Smith Point (SP) 29.543683 -94.785589 Gangs Bayou (GB) 29.258428 -94.9125 Vince Bayou (VB) 29.719444 -95.220481 Patrick Bayou (PB) 29.728233 -95.114031

Methods:

Oxygen was monitored using a Sensor Dish Reader to detect oxygen saturation in 24 well oxygen sensor plates (Precision Sensing GmbH). Briefly, fish were placed in each well, the wells were sealed, then oxygen content in the wells was recorded over time, as the fish consumed oxygen. Loss of equilibrium experiments were conducted in 1 ml glass vials stoppered with a ball of wax. Briefly, fish were put in the glass vials and sealed. After time, the fish began to lose equilibrium and lost the ability to right itself. As the fish began to turn upside down or sideways, the vial was inverted three times, with 5 seconds between each inversion. If the fish was unable to right itself after three inversions, the time was noted.

Instruments:

From Precision Sensing Gmbh - Germany SDR Sensor Dish Reader OxoDish - OD24 - 24-well plate for measuring oxygen on the SDR SDR v38 Software - Software for data acquisition

Individual Files: