Abstract:

This dataset is comprised of toxicity and biochemical and morphological assays that describe properties of resistance to dioxin-like compounds (DLCs) in four populations of Gulf killifish (Fundulus grandis) embryos. Two of these populations (Vince Bayou - VB and Paul's Bayou - PB) have been identified to be resistant to cardiac teratogenicity associated with exposure to polyaromatic hydrocarbons (PAHs.) Two reference populations were utilized for comparison (Gang's Bayou - GB and Smith Bayou -SP). Embyros were assessed for cross-resistance to pesticides (pyrethroid (permethrin), and carbamate (carbaryl)) and two model pro-oxidants (tert-butyl hydroquinone (tBHQ) and tert-butyl hydroperoxide (tBOOH)). Endpoints are mortality, degree of cardiac deformity, and the ethoxyresorufin-O-deethylase (EROD) assay to establish downregulation of cytochrome P450 1A as an indication of aryl-hydrocarbon receptor recalcitrance in resistant embryos. Morphological assays score the degree of heart deformities in embryos exposed to PCB-126.

Suggested Citation:

Elias Oziolor, Benjamin Dubansky, Warren Burggren and Cole Matson. 2016. Dataset for: Cross-resistance to pesticides and pro-oxidants in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds (DLCs). Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7513W6W

Publications:

Oziolor, E. M., Dubansky, B., Burggren, W. W., & Matson, C. W. (2016). Cross-resistance in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds. Aquatic Toxicology, 175, 222–231. doi:10.1016/j.aquatox.2016.03.019

Purpose:

The purpose was to determine if these populations are cross-resistant to both PAHs and oxidative stress, since there is much oxidative potential in the resistant populations (VB and PB.)

Data Parameters and Units:

Permethrin: Concentration (mass per unit volume); Mortality (percent mortality); Population (sampling population by site) tBHQ: tert-butyl hydroquinone (tBHQ) Concentration (mass per unit volume); Mortality (percent mortality); Population (sampling population by site) tBOOH: Model pro-oxidant tert-butyl hydroperoxide (tBOOH) Concentration (millimoles); Mortality (percent mortality); Population (sampling population by site) EROD: Dose (mL); EROD (fluorescence intensity of the urinary bladder, after background fluorescence was subtracted); Fluorescence intensity was box cox transformed for analysis. Population (sampling population by site) Cardiac Deformity: Dose (mL); Cardiac deformity index (scored quantitatively as 0=not deformed, 1=mildly deformed and 2=severely deformed); Population (sampling population by site); Rank Sampling site (abbreviation) latitude (decimal degrees) longitude (decimal degrees) Smith Point (SP) 29.543683 -94.785589 Gangs Bayou (GB) 29.258428 -94.9125 Vince Bayou (VB) 29.719444 -95.220481 Patrick Bayou (PB) 29.728233 -95.114031

Methods:

Methods were performed as described in Oziolor et al. 2016 (http://dx.doi.org/10.1016/j.aquatox.2016.03.019)

Individual Files: