Abstract:

Total mRNA profiles of 24, 48, 96 hpf mahi-mah larvae after slick and source oil exposure were generated by deep sequencing, in triplicate, using Illumina HiSeq2500.

Suggested Citation:

Elvis Genbo Xu, Ph.D.. 2016. Dataset for: Transcriptomic responses to Deepwater Horizon oil in mahi-mahi (Coryphaena hippurus) embryos reveals time and oil-dependent linkages between molecular initiating events and developmental toxicity. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7GX48ND

Publications:

Xu, E. G., Mager, E. M., Grosell, M., Pasparakis, C., Schlenker, L. S., Stieglitz, J. D., … Schlenk, D. (2016). Time- and oil-dependent transcriptomic and physiological responses to Deepwater Horizon oil in mahi-mahi (Coryphaena hippurus) embryos and larvae. Environ. Sci. Technol. doi:10.1021/acs.est.6b02205

Xu, E. G., Mager, E. M., Grosell, M., Hazard, E. S., Hardiman, G., & Schlenk, D. (2017). Novel transcriptome assembly and comparative toxicity pathway analysis in mahi-mahi (Coryphaena hippurus) embryos and larvae exposed to Deepwater Horizon oil. Scientific Reports, 7, 44546. doi:10.1038/srep44546

Xu, E. G., Mager, E. M., Grosell, M., Stieglitz, J. D., Hazard, E. S., Hardiman, G., & Schlenk, D. (2017). Developmental transcriptomic analyses for mechanistic insights into critical pathways involved in embryogenesis of pelagic mahi-mahi (Coryphaena hippurus). PLOS ONE, 12(7), e0180454. doi:10.1371/journal.pone.0180454

Purpose:

To help identify molecular mechanisms and pathways potentially involved in the developmental toxicity for fish exposed to Deepwater Horizon (DWH) oil, transcriptomic profiles in mahi-mahi (Coryphaena hippurus) embryos exposed to different DWH oils (source and artificially weathered oil) were evaluated at different critical windows of development using High Throughput Sequencing (HTS).

Data Parameters and Units:

total mRNA profiles, Run, BioSample, Sample name, MBases, Size (MBytes), Experiment, SRA Sample, transcript count, p-value, q-value, GeneID, concentration (g/l), slick = slick oil, mass = source HEWAF oil, Sample, Developmental Stage (24, 48 and 96 hours post fertilization (hpf)), treatment (control = 0 g/L, slick = 1 g/L, mass = 1 g/L)