Abstract:

The data collected in this study examined the influence of acute oil exposure on predator-prey dynamics (routine foraging activity and prey capture) in the larval red drum (Sciaenops ocellatus). Oil from the surface (OFS) during the Deepwater Horizon oil spill was used for the experiments. The data includes two main experiment settings: routine foraging activity and prey capture. Three oil doses (5, 10, 20% nominal concentrations) and a control were used in both assays. For routine foraging activity, approximately 21 dpf (days post fertilization) larvae were exposed to one of the treatments for 24 h and given no food to induce foraging behavior. After one-hour acclimation, activity was recorded for 15 min for the control, 5 and 10% oil doses. The videos were analyzed for total distance swam and thigmotaxis (percent of time spent near + away from the wall) using Ethovision, and for the area covered using ImageJ. Prey capture assays were performed with approximately 35 dpf larvae and 10 brine shrimp (Artemia nauplii). Time in seconds that took for the 1st, 5th, and 10th captures was recorded, as well as, the total number of prey items remaining after the 10-minute experiment. This dataset supports the publication: Rowsey, L. E., Johansen, J. L., Khursigara, A. J., & Esbaugh, A. J. (2019). Oil exposure impairs predator–prey dynamics in larval red drum (Sciaenops ocellatus). Marine and Freshwater Research. doi:10.1071/mf18263

Suggested Citation:

Rowsey, L.; Esbaugh, A.. 2019. Dataset for: Oil exposure impairs predator–prey dynamics in larval red drum (Sciaenops ocellatus). Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7S180V4

Publications:

Rowsey, L. E., Johansen, J. L., Khursigara, A. J., & Esbaugh, A. J. (2019). Oil exposure impairs predator–prey dynamics in larval red drum (Sciaenops ocellatus). Marine and Freshwater Research. doi:10.1071/mf18263

Purpose:

Examine the effect of oil exposure on larval red drum foraging ability and predator-prey dynamics.

Data Parameters and Units:

The dataset contains the 15-minutes videos (*.avi) for the foraging activity recorded after one-hour acclimation for the control, 5 and 10% oil doses. The dataset contains 3 additional excel: ELS_Forgaing_Activity_Data_Sheet.xls, Prey_Capture_Data_Sheets.xls, and Prey_Capture_&_Foraging_Acitivity_Oil_Comp.xlsx. ELS_Forgaing_Activity_Data_Sheet.xls: contains the test conditions table, the tank id dilution or stock code (tank id, start/end date and time, nominal concentration, replicate number and name initials of person collecting the information), WAF preparation information, water quality monitoring (tank id, date, time, period sampled [0h or 24h], water temperature [degrees C], pH [S.U.], D.O. [mg/L], salinity [ppt], total ammonia [micrograms], “-“ means no samples taken), sample inventory, and test performance with the information retrieved from the videos (tank id, GRIIDC ID (id used in the video filename), total distance swam [cm], body length [cm], thigmotaxis [time in zone, minutes]), relative distance (Distance/Body length divided by the average Distance/Body length), # of grids (number of squares in the arena in each video), 125 cm2 (percent of grids entered), the average (AVG) and SEM (standard error of the mean). Prey_Capture_Data_Sheets.xls: contains the test conditions table, the tank id dilution or stock code (tank id, start/end date and time, nominal concentration, replicate number and name initials of person collecting the information), WAF preparation information, water quality monitoring (tank id, date, time, period sampled [0h or 24h], water temperature [degrees C], pH [S.U.], D.O. [mg/L], salinity [ppt], total ammonia [micrograms]), sample inventory, and test performance with the prey-capture information (tank id, time in seconds that took for the 1st, 5th, and 10th captures and total number of prey eaten). Prey_Capture_&_Foraging_Acitivity_Oil_Comp.xlsx: contains the oil composition [µg/L] per sample for both experiments. ND= not determined.

Methods:

Larval red drum were placed into an open field test where foraging activity was measured and in a prey capture assay where the number of prey items captured in 10 minutes was observed.

Instruments:

The swimming activity was recorded with a uEye camera (IDS Imaging Systems, Obersulm, Germany), and the videos were analysed for thigmotaxis and total swimming distance using Ethovision XT (ver. 8.0, Noldus, Leesburg, VA, USA) tracking software.

Individual Files: