Abstract:

Two temperature assays were made: 1) a chronic temperature exposure where embryos were raised at 26° and 30°C degrees during 48 hours and 2) an acute temperature exposure, where embryos were raised at 25°C and acutely exposed at 25, 27 and 30°C. Chronic temperature effects on cardiac function and development were assessed only after 24 and 48 hpf. Acute temperature effects were assessed all along the development during 5 days.

Suggested Citation:

Perrichon, Prescilla. 2017. Temperature effects on developing heart in mahi-mahi. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7PR7T17

Publications:

Perrichon, P., Pasparakis, C., Mager, E. M., Stieglitz, J. D., Benetti, D. D., Grosell, M., & Burggren, W. W. (2017). Morphology and cardiac physiology are differentially affected by temperature in developing larvae of the marine fish mahi-mahi ( Coryphaena hippurus ) . Biology Open, bio.025692. doi:10.1242/bio.025692

Perrichon, P., Grosell, M., & Burggren, W. W. (2017). Heart Performance Determination by Visualization in Larval Fishes: Influence of Alternative Models for Heart Shape and Volume. Frontiers in Physiology, 8. doi:10.3389/fphys.2017.00464

Perrichon, P., Mager, E. M., Pasparakis, C., Stieglitz, J. D., Benetti, D. D., Grosell, M., & Burggren, W. W. (2018). Combined effects of elevated temperature and Deepwater Horizon oil exposure on the cardiac performance of larval mahi-mahi, Coryphaena hippurus. PLOS ONE, 13(10), e0203949. doi:10.1371/journal.pone.0203949

Purpose:

To examine the morphology and cardiac function changes following acute and chronic temperature exposures in early-life stages of mahi-mahi.

Data Parameters and Units:

Data file names include the parameters/species/developmental stage/temperature/and/or control/fish number (ex. Videos_Pictures/AcuteTemperatureAssay/Mahi_024h/T=25C/F002.jpg = a picture of 24 hpf Mahi fish, number F002, at Temperature 25 deg C taken during the acute temperature assay) Species, Condition, Developmental stages (hours post-fertilization), Temperature (deg C), Standard length (mm), yolk sac area (mm^2), yolk fluid area (mm^2), pericardial area (mm^2), Perimeter (mm), atrio-ventricular angle (°C), heart rate (beat/minute), stroke volume (nL), cardiac output (nL/minute), ventricular ejection fraction (%), Q10 (no unit), Video acquisition time (s), Heart beat number, Cardiac chamber (atrium or ventricle), Contractility state (End-Diastolic or End-Systolic), Major axe ellipse (mm), Minor axe ellipse (mm), Major Feret ellipse axe (mm), Minor Feret ellipse axe (mm), Volume ellipse (mm^3), Volume Feret ellipse (mm^3), Average volume, Intestinal area (mm^2), Fish name

Methods:

All data were collected from image and video acquisition. Morphometric measurement were made using Image J software with the freehand tool. Stroke volume is determined by the difference between the end-diastolic volume and the end-systolic volumes. Volumes are determined by the prolate spheroid equation. Cardiac output is the product between stroke volume and heart rate.

Instruments:

Nikon SMZ800 stereomicroscope coupled to a Fire-i400 or Fire-i530c digital camera (Unibrain, CA) + thermal microscope stage temperature controller (Book Industries, IL)

Individual Files: