Abstract:

Genome-wide mRNA and microRNA profiles of 48 hpf mahi-mahi (Coryphaena hippurus) larvae after treated with a series concentrations of slick as well as source oil were generated by next generation sequencing, in triplicate, using Illumina NextSEQ v2. All reads were pooled and assembled de novo using Trinity (version 2.2.0). Statistical bioinformatic analysis was further conducted by DAVID and Ingenuity Pathway Analysis to reveal dose-response biological effects of oil exposure.

Suggested Citation:

Xu, Genbo E. 2018. Dose-responsive mRNA and microRNA profiles in mahi-mahi (Coryphaena hippurus) larvae exposed to Deepwater Horizon oil. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7N29VHG

Publications:

Xu, E. G., Magnuson, J. T., Diamante, G., Mager, E., Pasparakis, C., Grosell, M., … Schlenk, D. (2018). Changes in microRNA–mRNA Signatures Agree with Morphological, Physiological, and Behavioral Changes in Larval Mahi-Mahi Treated with Deepwater Horizon Oil. Environmental Science & Technology, 52(22), 13501–13510. doi:10.1021/acs.est.8b04169

Purpose:

To help identify molecular regulatory mechanisms of developmental toxicity for fish exposed to Deepwater Horizon (DWH) oil.

Data Parameters and Units:

RNA seq/FASTQ, Sample name, Species, developmental stage, oil type, tissue, concentration (%), file name.

Methods:

Total mRNA and microRNA profiles of 48 hpf mahi larvae after slick and source oil exposure were generated by deep sequencing, in triplicate, using Illumina NextSEQ v2.

Instruments:

Illumina NextSEQ v2

Individual Files: