Abstract:

In this study, Siamese fighting fish (Betta splendens) were exposed to high energy water accommodated fraction (HEWAF) of crude oil in their diet in order to assess the effects of crude oil on behavioral parameters linked with the reproductive success of Siamese fighting fish. Thus, after 3 weeks of acclimation, fish were exposed to HEWAF for 4 weeks. Betta aggression display behaviors – opercular flaring, circling, darting and rapid fin movement were monitored. This dataset supports the publication: Bautista, N. M., Pothini, T., Meng, K., & Burggren, W. W. (2019). Behavioral consequences of dietary exposure to crude oil extracts in the Siamese fighting fish (Betta splendens). Aquatic Toxicology, 207, 34–42. doi:10.1016/j.aquatox.2018.11.025

Suggested Citation:

M. Bautista, N., Burggren, W.. 2019. Dataset for: Behavioral consequences of dietary exposure to crude oil extracts in the Siamese fighting fish (Betta splendens). Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7B56H6D

Publications:

Bautista, N. M., Pothini, T., Meng, K., & Burggren, W. W. (2019). Behavioral consequences of dietary exposure to crude oil extracts in the Siamese fighting fish (Betta splendens). Aquatic Toxicology, 207, 34–42. doi:10.1016/j.aquatox.2018.11.025

Purpose:

Determine if dietary exposure to crude oil has an effect on social and reproductive behaviors in the Siamese fighting fish

Data Parameters and Units:

The folder labeled “data” contains several excel files that contain the masses, specific growth rates, organ masses, nest surface area and presences, hematocrit data and aggressive behavior characterization of the various fish in the various experimental treatments (control, 50% HEWAF, 100% HEWAF) during either or both the acclimation and exposure periods. It also contains text readme files. The folder labeled “Photos_bubblenest” contains photographs used to estimate bubble nest characteristics at the specified date during the experiment when the variable was estimated. The excel file labeled “HEWAF_preparation_bettas” contains details on how HEWAF was made. It includes - Prep start date; Prep start time; Prep end date; Prep end time; WAF prep. method; Oil type; Water/diluent volume (L); Mass of dispersant added (mg); Mass of oil added (mg); Calculated nominal concentration (mg/L); Time of HEWAF transfer to sep. funnel; Time when drain sep. funnel; CEWAF stir start time; CEWAF stir end time/; settle start time; CEWAF settle end time; Filtered before use (y/n); Unfiltered PAH sample ID; Filtered PAH sample ID; Unfiltered VOC sample ID; Filtered VOC sample ID; Unfiltered DOSS sample ID; Filtered DOSS sample ID; Notes; Recorded by. The excel file labeled “ALS_Betta_fish” contains the analysis of polycyclic aromatic hydrocarbons (PAH) in control, 50% HEWAF and 100% HEWAF. Worksheet "AllData": contains information obtained by the laboratory ALS. The information is related to the concentrations of polycyclic aromatic hydrocarbons contained in the different dietary treatments used in this experiment. It includes - Client (Institution/Company that require the analysis); Project (Project in which the Institution/Company is working under); Service Request (Identification number assigned by ALS laboratory to this service); Sample (Control Food, 50% HEWAF, 100% HEWAF, Blank, Lab Control Sample, Duplicate Lab Control Sample; mg/Kg, Percent); Lab Code (designated identification number for each row and compound in the file); Sample Type; Date Collected (date when the sample was collected; MM/DD/YYYY); Date Received (date when the sample was received at the laboratory; MM/DD/YYYY); Date Extracted (date when the samples were extracted for analysis; MM/DD/YYYY); Date Analyzed (date when the results were obtained and analyzed; MM/DD/YYYY); Extraction Method (refers to how PAH were removed from solid); Method (analytical method); Matrix (whether it is solid or liquid); Basis (As received - the sample was processed as received at the laboratory); Units (measure units in which the results are given); Rings (This column was added by mistake and can be disregarded); Component (chemical compound found for each sample); Dilution Factor (Dilution factor used to analyze the sample); Reporting Limit (Concentration to which the compound is reported); Detection Limit (Detection limit of the equipment used to analyze the sample); Result (Estimated amount of the specific compound found in the sample); Result Notes (some description of results). Other Columns from W-AC (This analysis method does not provide results for this column, no data was obtained). Worksheet "Betta treatments": Chemical (Specific compound found in specific diet). Compounds highlighted with grey color are the specific 50 polycyclic aromatic hydrocarbons commonly measured in literature and the same reported in this study; Control Food (concentration of the specific compound in the control food, mg/Kg); 50% HEWAF (concentration of the specific compound in the 50% HEWAF food, mg/Kg); 100% HEWAF (concentration of the specific compound in the 100% HEWAF food, mg/Kg); BLANK (concentration of the specific compound in the Blank sample by ALS laboratory, mg/Kg). Note: Empty cells mean there is no value for the specific cell, it was not possible to estimate the value or assign to a group. Definition of the list of abbreviations and acronyms, and file naming system are provided in the readme files.

Methods:

Adult male betta fish (Betta splendens) were exposed to crude oil via diet. The food of the fish was sprayed with different concentrations of high energy water accommodated fractions solution (50% or 100% with oil load of 2 grams per liter of water), or water (control treatment). The exposure period lasted 4 weeks. The concentrations to which the fish were exposed are reported. Aggressive behavioral displays and nest-building behavior and characteristics of the nest (presence, surface area, depth and width) were assessed in a daily basis as indicators of reproductive success. Additionally, body and organ masses and hematocrit were estimated as indicators of fish health. Quantification of PAH-analytes was with the EPA method 8270D. For more information about these data see the associated publication mentioned in the abstract (Bautista, N. M. et al., 2019).

Instruments:

Quantification of PAH-analytes was with a gas chromatograph coupled with a mass spectrometer. A Nikon Coolpix AW130, 16 Mpx camera was used to quantify the different types of nest construction, photographs of each fish in its container and bubbles in either small groups or structured nests.

Individual Files: