Abstract:

Previous crude oil bioassays with larval mahi-mahi (C. hippurus) revealed an apparent dose-response effect of oil on the vertical orientation and distribution of larvae. This preliminary experiment sought to establish a method for quantifying these potential effects. This dataset focuses on measurements of vertical orientation and distribution of mahi-mahi larvae in 1 L glass beakers in response to increasing dilutions of 96 h slick (OFS) HEWAF exposure. Experimental setup and water quality data are also included.

Suggested Citation:

Mager, E., Grosell, M.. 2018. Effect of Oil Exposure on the Vertical Position of Larval Mahi-Mahi in the Water Column. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7SJ1J1H

Purpose:

To examine the effects of oil exposure on the vertical position and orientation of early life stage mahi-mahi in the water column.

Data Parameters and Units:

Tank ID dilution or stock code: C1-C4 = Control (0%) T1-T4 = Treatment (1%) T5-T8 = Treatment (4%) T9-T12 = Treatment (16%) Water Quality Monitoring.csv: Tank ID dilution or stock code; Date (DD-MMM-YY); Time (HH:MM); Period (Initial, 24hr, 48hr, 72hr, 96hr); Water temperature (deg C); pH; Dissolved oxygen (D.O., mg/L); Salinity (ppt); Total ammonia (uM); Notes; Recorded by WAF Preparation.csv: Prep date (MM/DD/YYYY); Prep time (HH:MM); Prep method (HEWAF-high energy water accommodated fraction); Oil type (OFS-oil from surface); Water/diluent volume (L); Mass of oil added (mg); Calculated nominal concentration (mg/L); Time (HH:MM) Test Performance Monitoring.csv: Tank ID; Date (DD-MMM-YY); Time (HH:MM); Number at test start; Number on surface; Number in 0-250 mL; Number in 250-500 mL; Number in 500-750 mL; Number in 750-1000 mL; Number vertically oriented; Number observed treatment mortalities; Number from non-treatment mortality; Number observed alive; Recorded by Tank ID or Stock Code.csv: Tank ID dilution or stock code; Start date (DD-MMM-YY); Start time (HH:MM); End date (DD-MMM-YY); End time (HH:MM); Nominal treatment concentration; Treatment units (%); Replicate number; Notes; Recorded by Test Conditions Table.csv: Species tested (C. hippurus); Life-stage/age (embryo); Oil type (OFS); WAF preparation method (HEWAF); Oil loading rate (1g/L); Nominal treatment (0-16%); Test chamber volume (mL); Test solution volume (mL); Number of replicates per treatment; Organisms per tank; Temperature (deg C); Salinity; Light source; Light intensity; Photoperiod (hours light/dark); Endpoints

Methods:

Vertical position was determined by assigning larvae to one of 4 vertical quadrants (~250 mL each) in a 1 L glass beaker.

Instruments:

YSI ProODO oxygen meter, PHM201 pH meter, refractometer, GC/MS-SIM

Individual Files: