Abstract:

This study was examining the influence of oil exposure long grow out studies. 15 individuals exposed to either control, low, or high oil dose were placed in a holding tank for 8 weeks. Length, weight, girth, height, and fin images were taken every two weeks to track growth. This was run in a resource rich and a resource patchy environment, where the feeding regime was altered based on the biomass of the tanks. Following the resource patchy study, when there was a significant difference between the specific growth rate of control and two oil doses, fish were either placed into an aerobic scope test or used for gene expression.

Suggested Citation:

Khursigara, A., Esbaugh, A.. 2019. Red drum mixed exposure long-term grow out study. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-0pyp-j104

Publications:

Khursigara, A. J., Johansen, J. L., & Esbaugh, A. J. (2021). The effects of acute crude oil exposure on growth and competition in red drum, Sciaenops ocellatus. Science of The Total Environment, 751, 141804. doi:10.1016/j.scitotenv.2020.141804

Purpose:

Investigate the impacts of oil exposure on long-term group competition.

Data Parameters and Units:

The dataset contains 1 folder of photos and 6 excel files (Oil Compostion.xls file; Resource Limited Image Analysis.xlsx; Grow Out Datasheets.xls; GCR1 gene expression analysis.xlsx; Fish Measurements.xlsx; Aerobic Scope Measurements.xls). The file “Oil Compostion.xls” contains measurements of polycyclic aromatic hydrocarbon compounds from various samples in the spreadsheet labeled “SuperDTA”. The values In SHEET1 are obtained from the spreadsheet “SuperDTA”. The file “Resource Limited Image Analysis.xlsx” contains final and initial fin scores: Caudal ( C), Dorsal (D), Left Pectoral (LP), and Right Pectoral (RP). The file “Grow Out Datasheets.xlsx” contains all various testing conditions, WAF (water accommodated fraction) preparation, water quality monitoring and sample inventory. The file “GCR1 gene expression analysis.xlsx” contains GCR 1 GenBank Accession Number and details on gene expression measurements. The file “Fish Measurements.xlsx” under the spreadsheets labeled “Resource Rich” and “Resource Limited” contains measurements to track growth under varying experimental conditions. The file “Aerobic Scope Measurements.xls” contains data on oxygen consumption. The measurements of height and width are in cm. The measurements of oxygen consumption and temperature are in mgO2/kg/hr and °C respectively. The measurements of the concentration of PAHs are in ug/L. Other relevant headers are pH (S.U.), dissolved oxygen (D.O., mg/L), Salinity (ppt), and Total ammonia (µM).

Methods:

5 PIT Tagged individuals were placed into 15 L of exposure for 24 h. After exposure, control, low oil, and high oil exposures were placed into an experimental tank together for 8 weeks. Length, mass, girth, and width every 2 weeks. Tanks were fed either 3% or 1% of the tank biomass to simulate resource rich and limited environments. After 8 weeks, resource limited survivors were split into 2 groups. Half were sacrificed and the brain, white muscle, kidney, liver, and heart were collected for gene expression. The other half were placed into an aerobic scope assay.

Instruments:

PIT Tagg, Loligo systems, Aquaresp.

Error Analysis:

All endpoints were analyzed with either One way or Two-way ANOVAs.

Individual Files: