Abstract:
This study examined the influence of oil exposure on dyad interactions in red drum. Differences in size, aerobic scope, and risk taking behavior between dominant and subordinate fish were examined to understand the characteristics that influence social hierarchy formation. Once it was established that aerobic scope was a key factor, interactions between individuals exposed to either 10 or 20% OFS HEWAF and an unexposed conspecific were observed. Exposure to 10% oil reduced an individual's ability to compete with an unexposed conspecific, however 20% exposure had no impact. This dataset supports the publication: Khursigara, A. J., Johansen, J. L., & Esbaugh, A. J. (2018). Social competition in red drum (Sciaenops ocellatus) is influenced by crude oil exposure. Aquatic Toxicology, 203, 194–201. doi:10.1016/j.aquatox.2018.08.011
Data Parameters and Units:
Test_Condition_Dryad Data Sheets.xlsx: Test Conditions Table worksheet. Tank ID Dilution or Stock Code worksheet: Tank ID Dilution or Stock Code (C1-24, T1-24), Start date (DD/MM/YYYY), Start time, End date (DD/MM/YYYY), Nominal Treatment Concentration (0, 10, 20), Treatment units (%), Replicate number. WAF Preparation worksheet: Prep start date, start time, end date, end time, prep. method, oil type, water/diluent volume (L), Mass of oil added (mg), Calculated nominal concentration (mg/L), time of HEWAF transfer to sep. funnel, time when drain sep. funnel. Water Quality Monitoring worksheet: Tank ID Dilution or Stock Code, Date, Time, Period, Water temp. (C), pH (S.U.), Salinity (ppt), Total ammonia (uM). Sample Inventory worksheet: sampling date, sampling time, tank ID dilution or stock code, number organisms per sample, unique ID number, sample ID, description of sample, storage location.
Raw_Aerobic_Scope Data.xlsx: Chamber #, wet weight (g), Resp. volume (mL), Chamber notes (fish #), Date & Time (MM/DD/YYYY/HH:MM:SS), Time (sec), Loop, Phase time (seconds), CH # MO2 (mgO2/kg/hr), CH # slope (%air sat./hr), CH # R^2, CH # max O2 (%air sat.), CH # min O2 (%air sat.), CH # avg O2 (%air sat.), CH # avg temp (°C).
Oil_Composition.xlsx: Original worksheet: Sample, Date Collected (MM/DD/YYYY), Date Received (MM/DD/YYYY), Date Extracted (MM/DD/YYYY), Date Analyzed (MM/DD/YYYY), Units (ug/L), Component, Dilution Factor, Reporting Limit, Detection Limit, Result.
Raw_Interactions_Data.xlsx: Size (cm), Fin score (Fin score was determined by comparing the initial and final images of the fins. Fish scored a 3 if an individual kept over 90% of fin area, 60-90% fin area received 2 points, 20-60% fin area received 1 point, and <20% received 0 points), Location (0 points if on the bottom of the tank, -5 for swimming at surface), Food (1 point to the individual who received the most food in the observation period), Attack (1 point for every aggressive interaction (or attack)
initiated), Avoidance (1 point for every avoidance behavior when an attack was not initiated).
Boldness_Videos: Video file naming convention: F #-# (fish being tested)_MM-DD-YY (date). Image file naming convention: Image Title: Tank (Letter of Tank used) E or N i or f D1/D2/C (fin). E: Elastomer, N: Non-elastomer, i: Initial, f: Final, D1: first dorsal fin, D2: second dorsal fin, C: caudal fin.
Dates on the folder titles indicate the day images were taken. Initial folders are the collection of images of the fish taken before the introduction into the one-on-one assay. Final folders are the collection of images of the fish taken following the one-on-one assay. Some dates only have a initial or final folders because reps were either not started (no initials) or taken down (no finals) that day.
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