Abstract:

The aim of this study was to investigate the effects of oil naturally irradiated on the survival, whole morphology and cardiac function in larval red drum (Sciaenops ocellatus). A range of concentrations of irradiated Source Oil B (SOB) were tested. Morphological and cardiac variables were assessed during the first developing 48 hours.

Suggested Citation:

Perrichon, P.. 2019. Irradiated oil exposure on morphology and cardiac function in red drum (Sciaenops ocellatus). Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7DZ06R4

Purpose:

To examine the morphology and cardiac function changes following oil exposure combined to two chronic temperatures exposures in early-life stages of red drum.

Data Parameters and Units:

The dataset contains 6 excel files which contain information on the chemistry of the medium, heart measurement, heart rate measurement, standard length of fish, survival, ultraviolet data and weather-temperature data. Dataset also contains 2 folders of videos and pictures, and text file explaining the naming convention of pictures and videos. The headers in the dataset are as follows- Radiation Type; Condition; Replicate Name; Fish Name; Chamber Name; Chamber State; Event Number; Area (mm^2); Perimeter; Major Axe (mm); Minor Axe (mm); Feret Axe (mm); Video Slice Number; MinFeret Axe (mm); Volume Ellipse (mm^3); Volume Feret (mm^3); Mean Vol (Ellipse+Feret) (mm^3); Components (ug/L); Control; Initial 60% HEWAF; Non-irradiated 60% HEWAF; Irradiated 60% HEWAF; Radiation Type; HEWAF Concentrations (microg/L); Replicate Name; Fish Name; Standard Length (mm); Date; Actual Time; UV Irradiation at 380nm (µWatts/cm/nm); Radiation Type; HEWAF Concentrations (microg/L);Replicate Name; Fish Name; Heart beat/min; Date; Time (CDT); Temp.; Heat Index; Dew Point; Humidity; Pressure; Visibility; Wind Dir; Wind Speed; Gust Speed; Precip Events; Conditions; Radiation Type; HEWAF concentration (microg/L); Replicate Name; Number at test start; Number hatched alive; and Number hatched dead.

Methods:

The high energy water accommodated fractions (HEWAFs) were generated at ambient temperature and prepared at a loading rate of 1 g of oil (Source Oil B) per liter of UV-sterilized seawater (35 ppt) and mixed in a Waring CB15 blender (Torrington, CT) at low speed for 30 s. Immediately after mixing, the solution was transferred to a glass separatory funnel for 1 h in the dark. After settling, the 90% lower portion of the solution was carefully drained and retained for subsequent use as 100% HEWAF. This solution was diluted to 60% with ambient seawater for the solar radiation process. UV radiation of HEWAF was by natural sunlight. Red drums were then exposed to different concentrations of SOB under laboratory conditions. All data were collected from image and video acquisition. Morphometric measurements were made using Image J software with the freehand tool. Stroke volume is determined by the difference between the end-diastolic volume and the end-systolic volumes. Volumes are determined by the prolate spheroid equation. Cardiac output is the product between stroke volume and heart rate. Total PAH characterization and quantification have been made by ALS Environmental (Kelso, WA, USA), according to standards of Deepwater Horizon Natural Resource Damage Assessment toxicity testing program.

Instruments:

Biospherical radiometer (BioSpherical Instruments, San Diego, CA) - Nikon SMZ800N stereomicroscope connected to a Nikon Digital DS U-3 camera.

Individual Files: