Abstract:

Cardiac output indices of stroke volume and heart rate were collected using doppler flow probes and doppler flow meter connected to a Powerlab data acquisition hardware connected to a Macintosh running Labchart v8.1.4 data acquisition software. All Labchart files were converted to .txt files. A readme file is included for each file. Water quality spread sheets were converted to .csv files.

Suggested Citation:

Nelson, Derek; Crossley, Dane. 2017. In vivo cardiac function during swimming in juvenile cobia (Rachycentron canadum) following crude oil exposure. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7N877W8

Publications:

Nelson, D., Stieglitz, J. D., Cox, G. K., Heuer, R. M., Benetti, D. D., Grosell, M., & Crossley, D. A. (2017). Cardio-respiratory function during exercise in the cobia, Rachycentron canadum : The impact of crude oil exposure. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 201, 58–65. doi:10.1016/j.cbpc.2017.08.006

Purpose:

To quantify cardiac output, heart rate, stroke volume, aerobic scope, maximum metabolic rate, minimum metabolic rate and oxergen consumption in exercising juvenile cobia following 24 hour exposure to 20% HEWAF of oil from surface.

Data Parameters and Units:

Tank ID Dilution or Stock Code; Date (DD-MMM-YY); Time (HH:MM); Period; Water Temperature (temp, deg. C); pH, dissolve oxygen (D.O., mg/L); salinity (ppt); total ammonia (uM); notes; recorded by; oil type; oil loading rate (g/L); Temperature (Temp, dec. C); Heart Rate indicated by Fh cham/chan 1 and 2 (HR, bpm); Flow (mL/min); SV (Flow Mean); Cardiac Output (SV*HR); Integral; Cardiac Output = volume of blood pumped by the heart per minute (CO, mL/min); Stroke Volume = volume of blood ejected by ventricle during each contraction indicated by VaortaFlow Cham1 and 2, ANIMAL 13 DC#9, COBIA#8 DC#4, COBIA #7 DC#5, ANIMAL 11 DC#8, ANIMAL 12 DEC#7, ANIMAL 16 DEC#7, ANIMAL 16 DC#10, (SWIM#1), and ANIMAL 15 DC#11 (SV, mL); PAH (ug/L); PAH depletion (%); WAF prep. method (HEWAF = high energy water accommodated fraction); Oil type (OFS = oil from the surface); Water/diluent volume (L); Mass of dispersant added (mg); Mass of oil added (mg); Calculated nominal concentration (mg/L); Nominal treatment concentration (% HEWAF); Life stage/age (size cm); Number of organisms at start; Number of treatment mortalities; Number of non-treatment mortalities; Number observed alive, Standard deviation indicated by Channel 5 and 6

Individual Files: