Abstract:
Larval mahi-mahi (Coryphaena hippurus) (7dph-10dph) were reared at the University of Miami- RSMAS where visual function was assessed using a flicker-fusion principle to monitor an optomotor response. Fish were placed in a glass cylinder with an opaque bottom, with a larger cylinder placed around the inner cylinder. The outer cylinder had alternating black and white stripes and driven by a 100 rpm motor, with a potentiometer assessing the speed of rotation. A GoPro (Hero 4+) was oriented above the chamber to view the fish’s behavior. Fish were preserved in Bouin’s solution for 24 hours, and transferred into a series of ethanol solution and placed in paraffin for histological work. Fish were sectioned by a microtome (5 µm sections) and a hematoxylin and eosin stain used. All slides were imaged on a Zeiss AX 10, and images analyzed using ImageJ software (v.1.43). This dataset includes videos of swimming mahi-mahi, microscope images of preserved and stained fish, and ocular measurements taken from images.
Suggested Citation:
Magnusson, Jason; Roberts, Aaron. 2017. Assessing visual acuity in a pelagic marine predator, mahi-mahi (Coryphaena hippurus). Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7X34VHD
Data Parameters and Units:
Tank ID, Fish number, Date (DD-MMM-YY), Time (HH:MM), Fish age (dph), Acclimation time (min), Spin direction (F=clockwise, R=counterclockwise), Time in chamber (min), Follow strip (yes/no), Maximum speed (RPM), Notes, Recorder initials, Video ID, Picture title, # cones/100 um, # ganglion cells/100 um, cones/ganglion, Lens diameter (um), Date analyzed (MM/DD/YYYY), Whole retina (um), Lens (um), Ganglion (um), Inner plexiform (um), Inner nuclear (um), Outer plexiform (um), Outer nuclear (um), Pigmented epithelium (um), Photoreceptor layer (um), Notes, Thickness (um), Area (um) This study is the first to look at larval fish acuity in the mahi-mahi, with studies conducted on vision in mahi-mahi primarily focusing on juvenile and adult stage. Significant differences were observed in retinal development from 7-10 dph larvae with significant tracking speeds observed during development. Data presented in this file focuses on video recordings of mahi-mahi larvae exhibiting an optomotor response with a common endpoint assessed- flicker fusion threshold. Mahi-mahi ranging from 7 to 10 day post hatch larvae were used, with two experimental groups run in subsequent days from each other. Fish were allowed to acclimate to the chamber prior to testing, with the first minute of video recorded as such. The length of each video varies, which is related to their responsiveness to the behavioral test. Videos were taken through the use of a GoPro with the files named according to fish age (dph), date recorded (DDMMMYY), fish number tested on that day, oil exposure concentration (0%), and whether or not a tracking response seen- noted as tracking (T) or not tracking (NT). Note: fish were not exposed to oil in this experiment
View Metadata