Abstract:

Cardiac intraventricular indices including stroke volume, heart rate, cardiac output, pressures, pressure volume relationships, stroke work, total polycyclic aromatic hydrocarbons (PAHs), systolic and diastolic cycle durations and contractility were collected for juvenile red drum (Sciaenops ocellatus) after 24 hour crude oil exposure to 10% HEWAF (high energy water accomodated fraction). Indices were collected using Scisence ADVantage Admittance PV system connected to Powerlab data acquisition hardware. This was connected to a Macintosh running Labchart v8.1.4 data acquisition software. All Labchart files were converted to .txt files. A readme file is also included. Water samples were sent to ALS for PAH concentration analysis.

Suggested Citation:

Nelson, Derek. 2016. In-situ cardiac function in juvenile red-drum (Sciaenops ocellatus) following 24 hour exposure to 10% HEWAF. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7PR7T2P

Purpose:

To quantify intraventricular indices of stroke volume, heart rate, cardiac output, pressures, pressure volume relationships, stroke work, systolic duration, diastolic cycle durations, and contractility in anesthetized juvenile red drum following 24 hour exposure to 10% HEWAF of oil from surface.

Data Parameters and Units:

Tank ID Dilution or Stock Code; Date (DD-MMM-YY); Time (HH:MM); Period, Water Temperature (temp, deg. C); pH, dissolved oxygen (D.O., mg/L); salinity (ppt); total ammonia (uM); notes; recorded by; Animal #; Treatment; Animal mass (kg); Pressure maximum (Pmax, kPa or mmHg); Pressure minimum (Pmin, kPa or mmHg); Pressure end diastolic (Ped or EDP, kPa); Pressure mean (Pmean, kPa); Max-Min Pressure (kPa); Systolic Duration (s); Diastolic Duration (s); Cycle Duration (s); Heart Rate (HR, bpm); Maximum change in pressure over time (dP/dt max or Max dP/dt, kPa/s or mmHg/s); Contractility Index (1/s); Minimum change in pressure over time (dP/dt min, kPa/s or mmHg/s); IRP Average dP/dt (kPa/s); Time constant of isovolumic relaxation (Tau, s); Pressure time index (kPa/s); Oil type (OFS = oil from the surface); Water/diluent volume (L); Mass of dispersant added (mg); Mass of oil added (mg); Calculated nominal concentration (mg/L); Time (HH:MM); Sample ID; Description of Sample; Storage location; Notes; Recorded by; Flow (mL/min); SV (Flow Mean); Stroke Volume = volume of blood ejected by ventricle during each contraction (SV, mL); Cardiac Output = volume of blood pumped by the heart per minute (CO, mL/min); Stroke Work = total work done by the ventricle to eject blood (SW, mmHg*mL); Pressure developed (Pdev, mmHg); Pressure end systolic (Pes, mmHg); Value of pressure at the point of maximum pressure over time (P@dP/dt max, mmHg); Maximum change in volume over time (dV/dt max, uL/s); Minimum change in volume over time (dV/dt min, mL/s); Volume max (Vmax, uL); Volume min (Vmin, uL); Volume end systolic (Ves, uL); Volume end diastolic (Ved, uL); Ejection Fraction (EF, %); Arterial elastance (Ea, mmHg/uL); Maximum Power (PowMax, mmHg*uL/s); Value of pressure at the point of maximum volume over time (P@dV/dt max, mmHg); Value of volume at the point of maximum pressure over time (V@dP/dt max, uL); Value of volume at the point of minimum pressure over time (V@dP/dt min, mL); PAH (ug/L); WAF prep. method (HEWAF = high energy water accomodated fraction); oil loading rate (g/L); Pressure (mmHg); Phase (degrees); Magnitude (uS); Cardiac Output (SV*HR); Integral; Heart rate flow (mL/min)

Individual Files: