Abstract:
This dataset contains chemistry data on otoliths of deepwater fishes collected aboard the R/V Point Sur cruises PS16-22 (DP03) and PS17-03 (DP04) in the Gulf of Mexico (GoM) from 2016-05-02 to 2016-08-19. Natural, chemical tracers in the otoliths of deep-pelagic taxa were used to assess ecological connectivity at two spatial scales: 1) within the Gulf of Mexico, and 2) across basins (i.e., the exchange between the GoM and adjacent waters of the western Atlantic Ocean [Bear Seamount, New England Seamount Chain], and the Caribbean). Chemistry data on otoliths of deepwater fishes in the GoM were collected as well as two classes of chemical tags (trace elements, stable isotope ratios) to examine variation in chemical signatures among distinct geographic locations. The dataset includes dates and location (latitude and longitude) of sample collection, the species of otoliths that were captured, and the averages and standard deviations of concentrations of various metals in tissue.
Suggested Citation:
Richards, Travis, Tracey Sutton, and Jay Rooker. 2020. Chemistry data on otoliths of deepwater fishes collected aboard the R/V Point Sur cruises PS16-22 (DP03) and PS17-03 (DP04) in the Gulf of Mexico from 2016-05-02 to 2016-08-19. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-npt8-8720
Data Parameters and Units:
Parameters: Species (Argyropelecus hemigymnus, Cyclothone obscura, Benthosema suborbitale, Lampanyctus alatus, Histrio histrio, Sternoptyx pseudobscura); Sample ID, date (dd-mon-yy); latitude and longitude (decimal degrees); and averages and standard deviations of concentrations of various metals in tissue - Mean Ca43 (cps (count per sec)), Stdev Ca43 (cps), Mean Ca_44 (ppm((part per million)), Stdev Ca_44 (ppm), Mean Mg_24 (ppm), Stdev Mg_24 (ppm), Mean Mn_55 (ppm), Stdev Mn_55 (ppm), Mean Li_7 (ppm), Stdev Li_7 (ppm), Mean Sr_88 (ppm), Stdev Sr_88 (ppm), Mean Ba_137 (ppm), Stdev Ba_137 (ppm), Mean Ba_138 (ppm), Stdev Ba_138 (ppm), Mean Cu_63 (ppm), Stdev Cu_63 (ppm), Mean Zn_66 (ppm), Stdev Zn_66 (ppm).
The dataset includes the cruise documentation for the R/V Point Sur cruises PS16-22 (DP03) and PS17-03 (DP04) for May (2016-05-02 to 2016-05-14), June (2016-06-07 to 2016-06-11), and August (2016-08-05 to 2016-08-19) led by chief scientists Tracy Sutton and Maelle Cornic respectively.
Correction note: In the data file "DEEPEND_Otolith Microchemistry Data.xlsx", the latitude and longitude coordinates for sample IDs Lala_21 and Lala_22 are incorrect (typos). The correct coordinates are:
For Lala_21 latitude = 27.5 (cell D101), longitude = -88 (cell E101)
For Lala_22 latitude = 27.5 (cell D102), longitude = -88 (cell E102)
Methods:
Prior to otolith extraction, each fish was weighed to the nearest 0.1 g and measured for standard length (SL) to the nearest 0.1 mm. Dissections to remove otoliths were performed using a Leica MZ75 stereomicroscope and Teflon coated forceps. Sagittal otoliths were extracted from the otic capsule following removal of the jaw, gill arches, and gill filaments. Once removed, sagittal otoliths were placed in trace element water, cleaned and allowed to dry thoroughly before storage in sterile 1.5 mL Eppendorf centrifuge tubes. Following extraction, whole otoliths were mounted to glass slides with Crystalbond™ thermoplastic glue with the distal surface flush against the glass slide and the proximal (sulcus acusticus) surface facing upward. Otoliths were individually polished using 800 and 1200 grit wet/dry sandpaper and 9.0 µm lapping film until a plane through the core of the otolith was obtained.
Instruments:
Otoliths were analyzed for 138Ba, 43-44Ca, 7Li, 24Mg, 55Mn, 88Sr and 66Zn using an Elemental Scientific NWR193UC (193 nm wavelength, <4 ns pulse width) laser system with a spot size of 25 µm and scan rate of 5µm/s coupled to an Agilent 7500ce inductively coupled mass spectrometer at the University of Texas at Austin.
View Metadata