Abstract:

The midwater column contains a wide diversity of cephalopods in the northern Gulf of Mexico. DNA sequence data (barcodes) have become a key tool to discover hidden biodiversity. We generated mitochondrial DNA barcode datasets based on the Cytochrome c oxidase I (COI), 16s, and 28s genes from cephalopods. These data were analyzed using phylogenetic methods to examine possible connectivity of 3 species (Vampyroteuthis infernalis, Pyroteuthis margaritifera, and Cranchia scabra) between two basins, the Gulf of Mexico and the area surrounding Bear Seamount in the northwestern Atlantic Ocean. These datasets were generated from cephalopods collected in the northern Gulf of Mexico on cruises DP01 through DP05 from May 2015 to May 2017.

Suggested Citation:

Amanda Sosnowki. 2018. Mitochondrial and nuclear sequences for phylogenetic analysis of three cephalopod species, northern Gulf of Mexico, May 2015 to May 2017. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7ZG6QQ9

Purpose:

To examine molecular genetics of three cephalopod species using both Sanger and ddRADseq techniques.

Data Parameters and Units:

The workbook contains 3 datasheets (COI,16s,28s) all have the following information: HBG Number, Unique Geneious Sequence ID, Ceph Jar Label, Phylum, Class, Order, Family, Genus, Species, COI Forward Primer, COI Reverse Primer, Cruise Info, Start Latitude (decimal degrees), Start Longitude (decimal degrees), Ending Latitude (decimal degrees), Ending Longitude (decimal degrees), Depth (m), ID on boat if changed, Genbank Accession #, Notes

Individual Files: