Dataset Available Remotely Hosted

Transcriptome data from hepatopancreas and gill tissues of Callinectes sapidus (blue crabs) exposed to surrogate crude oil in the laboratory and laboratory controls

Authors: Yednock, Bree K. , Sullivan, Timothy J., and Joesph K. Neigel
Published On: Sep 14 2015 18:43 UTC
DOI: https://doi.org/10.7266/N70863Q3
UDI: R3.x175.000:0002
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Project Information

Funded By:
Gulf of Mexico Research Initiative

Funding Cycle:
RFP-III

Research Group:
Continued Sampling of Blue Crab Larvae, Juveniles and Adults to Evaluate Oil Spill Effects on Population Connectivity and Adaptation, and Predict the Dynamics of Recovery

Point of Contact

Joseph Neigel
University of Louisiana at Lafayette / Department of Biology
jneigel@louisiana.edu

Theme keywords

blue crab, Callinectes sapidus, transcriptome, population genetics

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Abstract:

In 2010 blue crabs in the northern Gulf of Mexico were exposed to crude oil and chemical dispersants from the Deepwater Horizon oil spill. To characterize the blue crab transcriptome and identify genes that respond to oil exposure we sequenced transcriptomes from hepatopancreas and gill tissues of juvenile blue crabs exposed to a water-accommodated fraction of surrogate Macondo crude oil and compared those to transcriptomes from an unexposed control group. Illumina sequencing provided 42.5 million paired-end reads for the control group (3 individuals) and 44.9 million paired-end reads for the treatment group (4 individuals). These raw-reads are provided in the Bioproject 283615 deposited into NCBI (http://www.ncbi.nlm.nih.gov/bioproject/?term=283615). This dataset also includes data funded under RFP II R2.x214.000:0002.

Suggested Citation:

Yednock, Bree K. , Sullivan, Timothy J., and Joesph K. Neigel. 2015. Transcriptome data from hepatopancreas and gill tissues of Callinectes sapidus (blue crabs) exposed to surrogate crude oil in the laboratory and laboratory controls. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N70863Q3

Publications:

Yednock, B. K., Sullivan, T. J., & Neigel, J. E. (2015). De novo assembly of a transcriptome from juvenile blue crabs (Callinectes sapidus) following exposure to surrogate Macondo crude oil. BMC Genomics, 16(1). doi:10.1186/s12864-015-1739-2

Purpose:

This dataset was collected to evaluate the effects of oil spills on population connectivity and adaptation, and to predict the dynamics of population recovery.

Data Parameters and Units:

isolate type and raw transcriptomic sequence reads. The dataset contains 4 files 1 per sequence read archive (SRA): juvenile blue crab gill control,juvenile blue crab hepatopancreas control, juvenile blue crab gill tissue after oil treatment, juvenile blue crab hepatopancreas tissue after oil treatment.

Methods:

Treatment blue crabs were exposed to crude oil at a concentration of 2.5 ppm for 24 hours