Abstract:

We sampled fish from 10+ sites in and around Barataria Bay, Louisiana and from reference sites in Texas between August and October 2011. Fish were collected using minnow traps or seine at each field site. Sediment was collected using a sediment sampler at each site. Water samples at each site were collected into pre-cleaned Qorpak bottles. Data on phenotypic sex were collected by analysis of secondary sex characteristics and visual inspection of gonadal morphology. Fish weight was determined using a digital balance. Total and standard length were determined using a metric ruler. Blood was collected from euthanized fish by severing the caudal vein. Blood hematocrit was determined using a hematocrit centrifuge and standard hematocrit scale. Water quality measurements were collected using a YSI water quality meter.

Suggested Citation:

Carr, James. 2015. Fish tissue samples to assess reproductive and developmental abnormalities, Barataria Bay, LA, 2011. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N71834WX

Purpose:

The purpose was to collect samples in order to assess reproductive and developmental abnormalities using histopathological, biochemical and molecular approaches.

Data Parameters and Units:

WQ-- Site (place name) [Bay Jimmy, Bay Cray, Bay St. Denis, Oyster Bay, Boothville #1, Celebratory Bear Bayou, Grand Isle (Bay side), Grand Isle (Gulf side), Elmers Island, Joe's Landing Bait Shop, Port Sulphur #1, Sea Way Marina, Tydes Baitshop Leeville, Venice, Venice Bait Shop, Wagon Wheel, McFadden NWR], code (letters and numbers), Date (MM/DD/YYYY), Time (hh:mm am/pm), GPS latitude (degrees decimal minutes), GPS longitude (degrees decimal minutes), Water Temperature (degrees Celsius), Conductivity (mSi/cm), Salinity (PSU), Dissolved Oxygen (mg/L), Dissolved Oxygen (%), pH traps (count), water (yes or no), sediment (yest or no), Key to fish species-- Scientific Name [Arius felis, Bagre marinus, Cyptinodon variegatus, Dormitator maculatus, Evorthodus lyricus, Fundulus diaphanus, Fundulus grandis, Gambusia affinis, Gobiosoma bosc, Lagodon rhomboides, Lepomis gibbosus, Micropogonias undulatus, Mugil cephalus, Opsanus beta, Poecilia latipinna] Common Name [Hardhead catfish, Gaftopsail catfish, Sheepshead minnow, Fat sleeper goby, Lyre goby, Banded killifsh, Gulf killifish, Mosquito fish, Naked goby, Pinfish, Pumpkin seed sunfish, Croaker, Striped mullet, Gulf toadfish, Sailfin molly] [Meristic August 2011, Meristic September 2011, Meristic October 2011]-- Site ID (letter and numbers), Date (MM/DD/YYYY), status (alive or dead), collection (T=trap, D=dipnet, C=castnet, NA not applicable), Species (Abbreviated Scientific Name), Sex (M=male, F=female, IM= immanture, ?=unknown), BW Body Weight (g), TL Total Length (mm) SL Standard Length (mm), deposition (storage medium [RNA, RNA/formalin, Blood/formalin, formalin, Frozen]), Number of eggs collected (count), blood smear (yes), Notes, HCT (hematocrit (volume percentage (%) of red blood cells in blood))

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