Abstract:

This dataset covers GoMRI RFPII research performed at Louisiana Universities Marine Consortium (LUMCON) during 2016 in Dr. Chesney’s lab. Bay anchovy embryos (1dph) were exposed to toluene at range of concentrations. The larvae were assessed for overall survival and condition (malformations) immediately post exposure.

Suggested Citation:

Chesney, Edward. 2017. Comparison of toxicity of toluene to bay anchovy (Anchoa mitchilli) larvae. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7NC5ZMN

Purpose:

This dataset was compiled as part of a collaborative effort. Specifically, the study is designed to determine the effects of toxicity of single compounds of crude oil. Establishing the degree of post exposure effects on survival and the rate of developmental malformations was a key element of these studies.

Data Parameters and Units:

Experiment Filename: Data_Oil_BATolueneHatchingEmbryoExposure_48hrExposure_Oct272016 Worksheet 1 Exposure conditions Treatment label, Target species, Start Date (MM/DD/YYYY), Nominal Concentration (mg/L), Exposure Time (hrs), Replicate Number, Number Embryos at Start, Unhatched Dead embryos, Unhatched Alive embryos, Hatched Alive larvae, Hatched Dead larvae, Hatched Moribund larvae, Total Recovered, Final dissolved oxygen (DO, mg/L), Final Ammonia, Notes, Measured Toluene (ug/L), Estimated initial concentration (ug/L), Estimated Final Concentration (ug/L), Estimated mean concentration (ug/L) Worksheet 2 Malformations (post exp.) Treatment Label, Larval ID, Date (MM/DD/YYYY), Treatment Nominal Concentration (ug/L), Alive (A) or Moribund (M), Malformations (Post Exp., 1 = present): Spine, yolksac, Fin Fold, Eye Worksheet 3 General Information

Methods:

Exposure experiments: Bay anchovy embryos were placed in vials as pairs and allowed to hatch in the vials. Once the exposure time period was complete (48 hrs) the number and condition of the larvae or embryos were noted. After the exposures the survival and condition of remaining larvae in all treatments were evaluated. A set of vials were setup and never opened to measure the pattern of change in the chemistry at t0 vs t48 hrs.

Instruments:

Salinity was measured using a VitalSine ™ SR-6 Salinity Refractometer. Dissolved oxygen was measured using a YSI Professional Optical Dissolved Oxygen (ProODO™) Instrument. Ammonia levels were measured using an API Aquarium Pharmaceuticals Ammonia Test Kit. Toluene concentrations were estimated by PAH SVOC analysis by GC/MS.

Individual Files: