Abstract:

This dataset covers GoMRI RFP II research performed at Louisiana Universities Marine Consortium (LUMCON) during 2016 in Dr. Chesney’s lab. Bay anchovy larvae (3 and 4dph) were exposed to HEWAF at range of concentrations (0, 3, 10, 20%) for 2 hours. The larvae were assessed for overall post exposure survival and a subset of apparently healthy larvae placed as replicated groups in petri dishes filled with clean seawater and their behavior recorded from above in a shadowgraph immediately post exposure. A second group of larvae were exposed under the same conditions, moved and then held for 24hrs in clean seawater, their physical condition assessed 24 hrs post exposure and then a subset of apparently healthy larvae placed as replicated groups in petri dishes and their swimming behavior recorded.

Suggested Citation:

Chesney, Edward. 2017. Swimming performance of bay anchovy (Anchoa mitchilli) larvae after low dose short duration exposures to HEWAF. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7D798SK

Purpose:

The goal of the study was to assess immediate low dose short duration HEWAF exposure effects on swimming and the ability of the surviving first feeding bay anchovy larvae (3 and 4 DPH) to recover within 24 hrs.

Data Parameters and Units:

Excel File 1: BA Larvae_3%,10%HEWAF_2hr_7-22-16mortalitysummary Worksheet 1: Exposure Column labels: Tank Code, Trial Start Date, Trial End Date, Exposure Time (hrs), HEWAF Concentration (%), Exposure Duration (hr), Replicate Number, mL Seawater, mL HEWAF, Number Larvae (3 dph) start, Number Healthy. Number Dead, Number Moribund, Number Missing, Notes. HEWAF concentration is the percentage of the whole 1L volume composed of HEWAF (e.g. 10% = 100 mL HEWAF with 900 mL seawater to make 1L). Larvae were exposed 3 days post-hatch (dph). Worksheet 2: Fluorescence Column labels: 3% (RFU), 10% (RFU) Worksheet 3: GCMS Column labels: sample treatments, seawater control, 10% HEWAF, 10% HEWAF in ug/L Excel File 2: BA Larvae_3%,10%HEWAF_2hr_7_25_16mortalitysummary Worksheet 1: Exposures Column labels: Tank Code, Trial Start Date, Trial End Date, Exposure Time (hrs), HEWAF Concentration (%) , Exposure Duration (hr), Replicate Number, mL Seawater, mL HEWAF, Number Larvae (3 dph) start, Number Healthy, Number Dead, Number Moribund, Number Missing, Notes. HEWAF concentration is the percentage of the whole 1L volume composed of HEWAF (e.g. 10% = 100 mL HEWAF with 900 mL seawater to make 1L). Larvae were exposed 3 days post-hatch (dph). Worksheet 2: Fluorescence Column labels: 3% (RFU), 10% (RFU) Worksheet 3: GCMS Column labels: sample treatments, seawater control, 3% HEWAF, 10% HEWAF in ug/L Excel File 3: Filename: BA Larvae_3%,10%,20%HEWAF_2hr_24hrsGrow_8-1-16mortalitysummary Worksheet 1: Exposure conditions Column labels: Species, Life Stage, Tank Code, Trial Start Date, Exposure End Time, HEWAF Concentration (%), Exposure Duration (hrs), Replicate Number, mL Seawater, mL HEWAF, Notes. HEWAF concentration is the percentage of the whole 1L volume composed of HEWAF (e.g. 10% = 100 mL HEWAF with 900 mL seawater to make 1L). Larvae were exposed 3 days post-hatch (dph) in the presence of rotifers. Worksheet 2: Post Exposure Assessment Column labels: Tank Code, Time and Date Assessed, Number Larvae (3 dph) start, Number Healthy larvae, Number Dead larvae, Number Moribund larvae, Number Missing larvae, Initial Volume Clean Water First Transfer (mL), Final Volume Clean Water First Transfer (mL), Initial Volume Clean Water Second Transfer (mL), Final Volume Clean Water Second Transfer (mL), Number Larvae Transferred for 24 hr Grow-Out, Notes. Bay anchovy larvae were exposed 3 days post-hatch (dph). Worksheet 3: Post Exposure video information Column labels: Date, Treatment, Replicate, Species, Raw data file Name, Notes Worksheet 4: 24 hr. Post exposure Assessment Column labels: Tank Code, Time and Date Assessed Number Larvae after transfer, Number Healthy larvae, Number Dead larvae, Number Moribund larvae, Number Missing larvae, Final DO (%, mg/L), Final Ammonia (ppm), Final Temperature (C), Notes. The number of larvae at transfer are those that were healthy. Those were assessed for condition 24 hrs later prior to swimming them. Worksheet 5: 24 hr Post Video Information Column labels: Date, Treatment, Replicate, Species, Raw data file Name, Notes Worksheet 6: Fluorometry Column labels: 3% (RFU), 10% (RFU), 20% (RFU) Worksheet 7: GCMS Column labels: sample treatments, seawater control 0%, Ba 3% HEWAF, Ba 10% HEWAF, 20% HEWAF in ug/L Excel File 4: BA Larvae_3%,10%,20%HEWAF_2hr_24hrsGrow_8-8-16mortalitysummary Worksheet 1: Exposure Column labels: Tank Code, Trial Start Date, Exposure End Time, HEWAF Concentration (%), Exposure Duration (hr), Replicate Number, mL Seawater, mL HEWAF, Notes. HEWAF concentration is the percentage of the whole 1L volume composed of HEWAF (e.g. 10% = 100 mL HEWAF with 900 mL seawater to make 1L) and larvae were exposed 3 days post-hatch (dph). Worksheet 2: Instantaneous assessment Column labels: Tank Code, Date Assessed, Exposure duration (hrs), Percent HEWAF, Number Larvae (3 dph+) exposed, Number Healthy, Number Dead, Number Moribund, Number Missing, Number Larvae Transferred for 24 hr Grow-Out. Larvae were exposed 3 days post-hatch (dph). Worksheet 3: Instantaneous Video Information Column labels: Date, Treatment, Replicate, Species, File Name, Notes. HEWAF concentration is the percentage of the whole 1L volume composed of HEWAF (e.g. 10% = 100 mL HEWAF with 900 mL seawater to make 1L). Larvae were filmed with rotifers at a density of 2/mL. Worksheet 4: 24 hrs Post Assessment Column labels: Tank Code, Time and Date Assessed, Number Larvae start, Number Healthy, Number Dead, Number Moribund, Number Missing, Final DO (%, mg/L), Final Ammonia, Final Temperature Worksheet 5: 24 hrs Post Video Information Column labels: Date, Treatment, Replicate, Species, File Name, Notes. HEWAF concentration is the percentage of the whole 1L volume composed of HEWAF (e.g. 10% = 100 mL HEWAF with 900 mL seawater to make 1L). Larvae were filmed with rotifers at a density of 2/mL. Worksheet 6: Fluorescence Column labels: 3% HEWAF (RFU), 10% HEWAF (RFU), 20% HEWAF (RFU) Worksheet 7: GCMS Column labels: sample treatments, seawater control , BA 3% HEWAF, Ba 10% HEWAF, Ba 20% HEWAF in ug/L Excel file 5: Filename: Compiled bay anchovylarvae_swim_performanceData Worksheet1: 0% Immediate post exposure (controls) Column labels: 7-22-2016 3 DPH Bay anchovy larvae rep. A, 7-22-2016 3 DPH Bay anchovy larvae Rep. B, 7-25-2016 3 DPH Bay anchovy larvae Rep. A, 7-25-2016-2016 3 DPH Bay anchovy larvae Rep. B, 8-1-2016 3 DPH Bay anchovy larvae Rep A, 8-1-2016 3 DPH Bay anchovy larvae Rep. B, 8-8-2016 3 DPH Bay anchovy larvae Rep A, 8-8-2016 3 DPH Bay anchovy larvae Rep B. Velocity of fish in mm/s. Worksheet2: 3% HEWAF Immediate post exposure Column labels: 7-22-2016 3 DPH Bay anchovy larvae rep. A, 7-22-2016 3 DPH Bay anchovy larvae Rep. B, 7-25-2016 3 DPH Bay anchovy larvae Rep. A, 7-25-2016-2016 3 DPH Bay anchovy larvae Rep. B, 8-1-2016 3 DPH Bay anchovy larvae Rep A, 8-1-2016 3 DPH Bay anchovy larvae Rep. B, 8-8-2016 3 DPH Bay anchovy larvae Rep A, 8-8-2016 3 DPH Bay anchovy larvae Rep B. Velocity of fish in mm/s. Worksheet3: 10% HEWAFImmediate postexposure Column labels: 7-22-2016 3 DPH Bay anchovy larvae rep. A, 7-22-2016 3 DPH Bay anchovy larvae Rep. B, 7-25-2016 3 DPH Bay anchovy larvae Rep. A, 7-25-2016-2016 3 DPH Bay anchovy larvae Rep. B, 8-1-2016 3 DPH Bay anchovy larvae Rep A, 8-1-2016 3 DPH Bay anchovy larvae Rep. B, 8-8-2016 3 DPH Bay anchovy larvae Rep A, 8-8-2016 3 DPH Bay anchovy larvae Rep B. Velocity of fish in mm/s. Worksheet 4: 20% HEWAFImmediate postexposure Column labels: 8-1-2016 3 DPH BA larvae Rep. A, 8-1-2016 3 DPH BA larvae Rep B, 8-8-2016 BA larvae Rep. A, 8-8-2016 BA larvae Rep. B, 8-8-2016 BA larvae rep. A, 8-8-2016 BA larvae Rep. B. Velocity of fish in mm/s. Worksheet 5: 0% HEWAF 24 Hours Column labels: 8-2-2016 4 DPH BA larvae Rep. A, 8-9-2016 BA Larvae Rep. A, 8-9-2016 BA Larvae Rep. B. Velocity of fish in mm/s. Worksheet 6: 3% HEWAF 24 Hours Column labels: 8-2-2016 4 DPH BA larvae Rep. A, 8-9-2016 BA Larvae Rep. A, 8-9-2016 BA Larvae Rep. B. Velocity of fish in mm/s. Worksheet 7: 10% HEWAF 24 Hours Column labels: 8-2-2016 4 DPH BA larvae Rep. A, 8-9-2016 BA Larvae Rep. A, 8-9-2016 BA Larvae Rep. A, 8-9-2016 BA Larvae Rep. B. Velocity of fish in mm/s. Worksheet 8: 20% HEWAF 24 Hours Column labels: 8-2-2016 3 DPH BA Larvae Rep. A, 8-2-2016 3 DPH BA Larvae Rep. B, 8-9-2016 3 DPH BA Larvae Rep. B. Velocity of fish in mm/s. Worksheet 9: swim velocity averages Column labels: Statistic, 0% (control) Immediate, 3% HEWAF Immediate, 10% HEWAF Immediate, 20% HEWAF Immediate, 0% (control) 24 Hrs Post, 3% HEWAF 24 Hrs Post, 10% HEWAF 24 Hrs Post, 20% HEWAF 24 Hrs Post

Methods:

Exposure experiments: Bay anchovy larvae were placed in beakers with different concentrations of HEWAF. After 2 hr exposures they were placed in clean seawater and then into petri dishes and then placed in a shadowgraph where their behavior was recorded in the presence of prey (rotifers). This was repeated with a second group of larvae where after 2 hrs of exposure they were placed in clean seawater, held for 24 hrs, mortality assessed and then small groups placed in petri dishes and then their behavior recorded. Swimming behavior was assessed from the videos in a frame by frame analysis of randomly selected sequences.

Instruments:

Salinity was measured using a VitalSine ™ SR-6 Salinity Refractometer. Dissolved oxygen was measured using a YSI Professional Optical Dissolved Oxygen (ProODO™) Instrument. Ammonia levels were measured using an API Aquarium Pharmaceuticals Ammonia Test Kit. HEWAF concentrations were confirmed by fluorescence measures and analyses by GC/MS.

Individual Files: