Abstract:

Data generated by experiments designed to assess antioxidant responses resulting from 24 or 96 hr exposures to Corexit 9500 or DOSS. Parameters measured include levels of reduced glutathione levels (GSH), and oxidized glutathione (GSSG), both expressed as nM/mg wet weight of larvae, reactive oxygen species (ROS) measured as the increase in fluorescein resulting from the oxidation of dihydrodichlorofluorescein diacetate, and lipid peroxidation, measured using the TBARS assay which monitors increases in malondialdehyde (MDA) levels, both expressed as uM/mg protein of larval homogenates. Activities of a number of antioxidant cycle related enzymes are also provided including: glutathione-S-transferase (GST), glutamate-cystein-ligase (GCL), glutathione reductase (GR), glutathione peroxidase (GPx), all expressed as nM/min/mg protein of larval homogenate. Superoxide dismutase (SOD) is reported as units (U)/mg larval homogenate (each unit is equivalent to the amount of enzyme required to catalyze conversion of 1 uM of substrate/min). CYP1A activity was measured following the production of resorufin per larva in 24 hrs.

Suggested Citation:

Anne McElroy and Subham Dasgupta. 2017. Antioxidant responses in sheepshead minnow larvae exposed to Corexit 9500 or DOSS. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N75M643F

Publications:

Dasgupta, S., Choyke, S., Ferguson, P. L., & McElroy, A. E. (2018). Antioxidant responses and oxidative stress in sheepshead minnow larvae exposed to Corexit 9500 ® or its component surfactant, DOSS. Aquatic Toxicology, 194, 10–17. doi:10.1016/j.aquatox.2017.10.010

Purpose:

The goal of this study was to determine modes of cellular response following exposure to Corexit 9500 or its major component, DOSS in sheepshead minnow larvae.

Data Parameters and Units:

Glutathione (GSH) - nM/mg wet weight of larvae Oxidized glutathione (GSSG) - nM/mg wet weight of larvae Reactive oxygen species (ROS) - uM/mg protein of larval homogenates Lipid peroxidation - uM/mg protein of larval homogenates Glutathione-S-transferase (GST) - nM/min/mg protein of larval homogenate Glutamate-cystein-ligase (GCL) - nM/min/mg protein of larval homogenate Glutathione reductase (GR) - nM/min/mg protein of larval homogenate Glutathione peroxidase (GPx) - nM/min/mg protein of larval homogenate Superoxide dismutase (SOD) - (U)/mg larval homogenate (each unit is equivalent to the amount of enzyme required to catalyze conversion of 1 uM of substrate/min) Concentrations of Corexit 9500 - mg/L DOSS - mg/L Time - hours

Methods:

Reactive oxygen species (ROS) measured as the increase in fluorescein resulting from the oxidation of dihydrodichlorofluorescein diacetate. Lipid peroxidation measured using the TBARS assay. CYP1A activity was measured following the production of resorufin per larva in 24 hrs.

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