Abstract:

This dataset describes how cyclodextrin-promoted energy transfer occurs for a broad range of aromatic toxicants in multiple complex environments, including plasma, coconut water, and breast milk. Up to 168% energy transfer efficiencies were observed in phosphate buffered saline, and up to 39% energy transfer efficiencies were observed in complex media. Preliminary efforts towards rational tuning of the fluorophore emission signal for a particular analyte are also reported.

Suggested Citation:

Mindy Levine and Nicole Serio. 2016. Dataset for: Cyclodextrin-​promoted energy transfer for broadly applicable small-​molecule detection. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7QZ280N

Publications:

Serio, N., Chanthalyma, C., Prignano, L., & Levine, M. (2014). Cyclodextrin-promoted energy transfer for broadly applicable small-molecule detection. Supramolecular Chemistry, 26(10-12), 714–721. doi:10.1080/10610278.2013.860226

Purpose:

To investigate cyclodextrin-promoted energy transfer for a broad range of aromatic toxicants in multiple environments.

Data Parameters and Units:

Compound, excitation wavelength (nm), emission spectrum (nm), concentration (uM), control ratio, energy transfer percentage (ET)

Methods:

General procedure for energy transfer experiments: γ-cyclodextrin hydrate (CAS: 91464-90-3) was obtained from Sigma-Aldrich, and dissolved in phosphate buffered saline (PBS) at pH 7.4 at a 10 mM concentration. All analytes were dissolved at a concentration of 1 mg/mL in tetrahydrofuran (THF). Fluorophore solutions were made as follows: BODIPY 20: 0.1 mg/mL in THF Rhodamine 21: 0.1 mg/mL in THF 2.5 mL of the cyclodextrin solution was transferred to a quartz cuvette, and 20 µL of the analyte solution was added via micropipette. The absorbance and fluorescence spectra of the solution were recorded. The fluorophore was then added (100 µL), and the absorbance and fluorescence spectra were recorded. The final concentrations of each analyte and fluorophore are shown in Table S1. For each combination, two fluorescence spectra were recorded: the fluorescence from excitation of the analyte and the fluorescence spectra from excitation of the fluorophore. The excitation wavelengths were chosen to be as close as possible to the maximum wavelength of absorption, without significantly truncating the emission spectrum.

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