Abstract:

We demonstrate that the use of phosphate buffer vs. phosphate buffered saline dramatically effects the ability of cyclodextrins to extract PAHs from complex oils, providing important insight into the mechanism behind such extractions. This dataset contains energy transfer efficiencies and analyte comparisons of oil spill oil and tar ball oil.

Suggested Citation:

Mindy Levine. 2016. Energy transfer efficiencies and analyte comparisons used to detect the extraction of polycyclic aromatic hydrocarbons (PAHs) from oils. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7M906K6

Publications:

Serio, N., & Levine, M. (2015). Solvent effects in the extraction and detection of polycyclic aromatic hydrocarbons from complex oils in complex environments. Journal of Inclusion Phenomena and Macrocyclic Chemistry, 84(1-2), 61–70. doi:10.1007/s10847-015-0583-y

Serio, N., Chanthalyma, C., Peters, S., Levine, D., & Levine, M. (2014). 2-Hydroxypropyl beta-cyclodextrin for the enhanced performance of dual function extraction and detection systems in complex oil environments. Journal of Inclusion Phenomena and Macrocyclic Chemistry, 81(3-4), 341–346. doi:10.1007/s10847-014-0460-0

Purpose:

To determine the solvent system necessary for optimal extraction efficiencies

Data Parameters and Units:

All data quantified with fluorescence spectroscopy. buffer for paper-file folder Oil spill oil and tar ball calculated analyte comparisons (analyte comp) for three analytes. Energy transfer efficiencies in the aqueous layer using a phosphate buffer (fluorescence emission from excitation at the analyte wavelength divided by the fluorescence emission from direct fluorophore excitation) (%). 2-HPCD (2-hydroxypropyl-β-cyclodextrin); γ-CD or gcd (γ-cyclodextrin); et in water: energy transfer in the aqueous layer; Analyte compounds 1: Anthracene, 2: Benzo[a]pyrene, 3: Pyrene. Complex oils buffer project-file folder Oil [oil spill oil or tar ball] + analyte + cyclodextrin derivative. Oil spill oil and tar ball energy transfer efficiencies in the aqueous and oil layer, control ratios, analyte comparisons, and bodipy comparisons using a phosphate buffer. 2hpcd (2-hydroxypropyl-β-cyclodextrin); γ-CD or gcd (γ-cyclodextrin); pb (phosphate buffer); Analyte compounds: anthra (anthracene), benzo (Benzo[a]pyrene), pyrene; fluoro (fluorophore used in controls); wavelength (nm); aq (aqueous layer); et in oil (energy transfer in oil layer); et in water (energy transfer in aqueous layer). Seawater section all-file folder Analyte comparisons and energy transfer efficiencies of oil spill oil and tar ball oil in crude seawater collected from Narragansett Bay. Analytes doped into oil-water mixtures. aq (aqueous layer); pbs (phosphate buffered saline); acd (alpha-cyclodextrin); bcd (beta-cyclodextrin); mebcd (methyl-beta-cyclodextrin); 2hpcd (2-hydroxypropyl-β-cyclodextrin); gcd (γ-cyclodextrin); Analyte compounds: anthra (anthracene), benzo (Benzo[a]pyrene), pyrene; et in water (energy transfer in aqueous layer). revised griidc solvet effects.docx-summary tables of analyte comparisons and energy transfer efficiencies.

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