Abstract:

We measured the dynamic interfacial tension of Cerato-ulmin (CU) dipsersions at air-water and squalane-water interfaces. CU stock solution was prepared in aqueous solutions at a concentration of 0.2mg/mL. Experimental trials used CU solution at concentrations of 1 ug/mL and 2 ug/mL. Cerato-ulmin was studied using a microtensiometer. An inverted light microscope was used to image bubbles or drops. Both static and dynamic experiments were performed, and both air-water and oil-water interfaces were measured (the oil was squalane). This dataset reports interfacial tension and drop radius measurements of CU membranes in air-water and squalane-water interfaces.

Suggested Citation:

Paul Russo. 2017. Interfacial tension and radius measurements of Cerato-ulmin hydrophobin membranes at air-water and oil-water interfaces. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N728061Q

Purpose:

The aim of this research was to assess the strength of biofilms of a natural protein that nature uses to clean oil spills.

Data Parameters and Units:

Figure 2.opj - plot of interfacial tension (mN m^-1), time (seconds), CU concentration at air-water interface (1 ug/mL, 2 ug/mL) Figure 3.opj - time (seconds), interfacial tension of CU at air-water interface (mN m^-1), early rinse (deionized water rinsed of 1 ug/mL of CU at air-water interface at 1600 seconds), late rinse (deioinized water used to rinse of 1 ug/mL of CU at air-water interface at 4200 seconds) Figure 4.opj - plot of interfacial tension (mN m^-1), radius (um), time (seconds), of 2 ug/ML of CU at air-water interface during oscillation (omega = 1.8 rad/s) Figure 5.opj - dilational modulus (mN m^-1), time (seconds), CU concentration at air-water interface (1 ug/mL, 2 ug/mL) Figure 6.opj - interfacial tension 2 ug/mL of CU at squalane-water interface (mN m^-1), time (seconds), pre-exposed (microtensiometer pre-exposed to dilute CU solution; measurement with 46 um interfacial curvature), post-exposed (microtensiometer post-exposed to dilute CU solution; measurement with 73 um interfacial curvature) Figure 7.opj - interfacial tension of 2 mg/mL CU (mN m^-1) at squalane-water and air-water interface, time (seconds) Figure 8.opj - plot of interfacial tension (mN m^-1), radius (um), time (seconds) of 2 ug/mL of CU at squalane-water interface during oscillation Figure 9.opj - dilational modulus (mN m^-1), time (seconds) of 2ug/mL of CU at squalane-water interface

Methods:

.opj files can be opened with Origin viewer.

Individual Files: