Abstract:
We conducted a laboratory experiment to examine how the decomposition of belowground particulates is enhanced, or not, by different mixtures of oil, nitrogen, or phosphorus acting individually or synergistically. The experiment was conducted in 3.8 L sampling chambers producing varying quantities of gas under anaerobic conditions.
Suggested Citation:
Turner, Eugene R.. 2016. Dataset for: The effects of N, P and crude oil on the decomposition of Spartina alterniflora belowground biomass. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N78050JZ
Data Parameters and Units:
Experiment a: Plant (Spartina alterniflora); Treatment (DW salt = distilled water + salt, DW salt oil = distilled water + oil + salt, DWSOIL NP = distilled water + oil + Nitrogen + Phosphorus, DWS N = distilled water + Nitrogen, DWS P = distilled water + Phosphorus, DWS NP = distilled water + Nitrogen + Phosphorus); Replicate (rep, 1/2/3); Bottle number; Time (hr, min, AM/PM); Date (Month, Day, Year); Temperature (Temp, deg C); Water volume (mL); pH; Cumulative water volume (CUM, mL). Experiment data part b: Plant (Spartina alterniflora); Treatment ((DW salt = distilled water + salt, DW salt oil = distilled water + oil + salt, DWSOIL NP = distilled water + oil + Nitrogen + Phosphorus, DWS N = distilled water + Nitrogen, DWS P = distilled water + Phosphorus, DWS NP = distilled water + Nitrogen + Phosphorus); Replicate (rep, 1/2/3); Bottle number; Experimental Day (9= August 9th); Water volume (mL); Cumulative water volume (CUM, mL). Sheet4: LSU/RCAT ID#; Field ID# (40, 41, 42 are control replicates before the experiment, 43, 44, 45 are treatment replicates after the experiment); Initial Water Volume (mL); Final Extract Volume (mL); Alkane Analyte Concentration (ug/L); nC-10 Decane nC-11 Undecane nC-12 Dodecane nC-13 Tridecane nC-14 Tetradecane nC-15 Pentadecane nC-16 Hexadecane nC-17 Heptadecane Pristane nC-18 Octadecane Phytane nC-19 Nonadecane nC-20 Eicosane nC-21 Heneicosane nC-22 Docosane nC-23 Tricosane nC-24 Tetracosane nC-25 Pentacosane nC-26 Hexacosane nC-27 Heptacosane nC-28 Octacosane nC-29 Nonacosane nC-30 Triacontane nC-31 Hentriacontane nC-32 Dotriacontane nC-33 Tritriacontane nC-34 Tetratriacontane nC-35 Pentatriacontane Total Alkanes % Surrogate Recovery 5 Alpha Androstane Aromatic Analyte Concentration (ng/L); Surrogate Corrected (ng/L);Naphthalene C1-Naphthalenes C2-Naphthalenes C3-Naphthalenes C4-Naphthalenes Fluorene C1-Fluorenes C2-Fluorenes C3- Fluorenes Dibenzothiophene C1-Dibenzothiophenes C2-Dibenzothiophenes C3- Dibenzothiophenes Phenanthrene C1-Phenanthrenes C2-Phenanthrenes C3-Phenanthrenes C4-Phenanthrenes Anthracene Fluoranthene Pyrene C1- Pyrenes C2- Pyrenes C3- Pyrenes C4- Pyrenes Naphthobenzothiophene C-1 Naphthobenzothiophenes C-2 Naphthobenzothiophenes C-3 Naphthobenzothiophenes Benzo (a) Anthracene Chrysene C1- Chrysenes C2- Chrysenes C3- Chrysenes C4- Chrysenes Benzo (b)Fluoranthene Benzo (k) Fluoranthene Benzo (e) Pyrene Benzo (a) Pyrene Perylene Indeno (1,2,3 - cd) Pyrene Dibenzo (a,h) anthracene Benzo (g,h,i) perylene Total Aromatics % Surrogate Recovery Phenanthrene d-10
Methods:
Thirty-three g of dried belowground biomass was placed in a 3.8 L incubation chamber and various combinations of N, P and/or oil added. Gas production generated within the incubation chamber displaced water up the vertical tube embedded in the substrate. The volume displacement is the metric of change and measured as ml gas. Gas production was trapped within the incubation chamber and the displaced water was forced up the vertical tube embedded in the substrate. A relief valve was periodically opened to drain the water in the tube to bring the water level back to zero. Gas production was estimated as being equal to the water volume displaced. The data are presented as means ± 1 std. error (SE; n=3). The analysis identified 28 alkanes and 43 aromatic hydrocarbons and their respective alkyl homologs using GC/MS-SIM (gas chromatography/mass spectrometry in selective ion monitoring mode), including the normal and branched saturated hydrocarbons (from C10 to C35), the one- to five-ringed aromatic hydrocarbons and their C1 to C4 alkyl homologs, as well as cyclic biomarker compounds like the hopanes, steranes and triaromatic steroids (SIM ions 191, 217, 218, and 231 eluting between C23 and C31). The water samples were analyzed using accepted standard operating and QA/QC procedures to prevent contamination and avoid sample degradation. The samples were extracted with dichloromethane (DCM) and spiked with surrogate standards to achieve a final extract concentration of 20 µg mL-1. The concentrations of the target PAHs were determined by an internal standard method and response factor calculated from a 5-point calibration curve using a commercially-available standard containing the normal alkanes from n-C10 through n-C35 and the parent PAH analytes of interest. The MS detector was tuned to PFTBA (perfluorotributylamine) before each set of analyses. If any of the tune parameters (e.g., percent air/water, peak abundances and ratios) were significantly different from before tune parameter values, then the instrument was checked for errors and then returned to normal operating conditions. A daily calibration standard and blank were analyzed with each sample batch to verify proper instrument performance. If the daily standard indicated instrumental problems, then no further analytical work was performed until the instrument was restored to good operating condition. The identities of all analytes were established using retention times and full scanning mass spectral data. The spectral data were processed by Chemstation Software (Agilent Technologies).
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