Abstract:

Collection data, micrographs, Prorocentrum Cob1 data and Haptophyta 18S and tufA data in support of the study: Krayesky-Self, S.; Schmidt, W.E.; Phung, D.; Henry, C.; Sauvage, T.; Camacho, O.; Felgenhauer, B.E.; Fredericq, S. (2017). Eukaryotic Life Inhabits Rhodolith-forming Coralline Algae (Hapalidiales, Rhodophyta), Remarkable Marine Benthic Microhabitats. Scientific Reports, 7, , 45850. doi:10.1038/srep45850. Environmental factors obtained on the collection cruises are available in GRIIDC datasets: R1.x139.145:0001, R1.x139.145:0008, R1.x139.145:0009 and R1.x139.145:0013.

Suggested Citation:

Fredericq, Suzanne. 2017. Dataset for: Eukaryotic Life Inhabits Rhodolith-forming Coralline Algae (Hapalidiales, Rhodophyta), Remarkable Marine Benthic Microhabitats. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7XG9PKX

Publications:

Krayesky-Self, S., Schmidt, W. E., Phung, D., Henry, C., Sauvage, T., Camacho, O., … Fredericq, S. (2017). Eukaryotic Life Inhabits Rhodolith-forming Coralline Algae (Hapalidiales, Rhodophyta), Remarkable Marine Benthic Microhabitats. Scientific Reports, 7, 45850. doi:10.1038/srep45850

Purpose:

To investigate interior functions of rhodoliths in the Gulf of Mexico.

Data Parameters and Units:

File: R1.x139.000-0005_Cob1_sub2.txt Accession number and gene sequences for the mitochondrial-encoded gen Cob1 in the Prorocentrum. File: R1.x139.000-0005_18S-tufA_sub2.txt Parameter Descriptions: Accession number and gene sequences for the nuclear small subunit 18S and chloroplast-encoded gene tufa in the Haptophyta. File: R1.x139.000-0005_Collection data_sub2.txt Parameter Descriptions: Collection information for deepwater rhodolith collections representing two undescribed species of crustose coralline red algae newly determined and annotated species as well as additional samples analyzed in present study: Lithothamnion Sample (user defined sequential numbers assigned to samples); LAF Identification Number (Voucher number assigned upon deposit at the Algal Herbarium of the University of Louisiana at Lafayette); Preservation mode (disposition of collected samples for later processing: mesocosm use or long-term preservation for subsequent DNA analysis); Location (general descriptive associated with collection location); Latitude (latitude of sample collection, decimal degrees); Longitude (longitude of sample collection, decimal degrees); Depth (collection depth, meters); Collection Date (Date samples were acquired, MM/DD/YYYY). File: R1.x139.000-0005_Micrographs Windows folder containing 17 various micrographs of endolithic life history stages of Prorocentrum lima dinoflagellates and rhodolith-forming coralline red algae Photo_1.jpg=Rhodolith habitat Photo_2.BMP=SEM micrograph of a cross section through Lithothamnion sp. 1 Photo_3.tif=SEM micrograph of Lithothamnion sp. 1 perithallial cells containing endolithic inclusions that display dinoflagellate characteristics Photo_4.tif=SEM micrograph of Lithothamnion sp. 1 perithallial cells containing endolithic inclusions that display dinoflagellate characteristics Photo_5.tif=SEM micrograph of Lithothamnion sp. 1 perithallial cells containing endolithic inclusions that display dinoflagellate characteristics Photo_6.tif=Dinoflagellate stages showing blebs, i.e. organized membrane projections Photo_7.tif=Fluorescent micrograph of a semi-thin (perithallial and upper epithallial cell layers) section through a rhodolith Photo_8.tif=Light micrograph of deeper cell layers (lower perithallil cells) of a rhodolith Photo_9.tif=Light micrograph of isolated endolithic cellular inclusions stained with Osmium Tetroxide and Methyl Blue Photo_10.tif=Transmission electron (TEM) micrograph showing a layer of extracellular matrix surrounding cell Photo_11.tif=Transmission electron (TEM) micrograph highlighting pusules in Photo_10 cell Photo_12.tif=Transmission electron (TEM) micrograph highlighting mitochondria in Photo_10 cell Photo_13.tif=Transmission electron (TEM) micrograph highlighting the condensed chromosomes in the dinokaryon Photo_14.tif=Cultures of non-flagellated cells isolated from within the rhodolith shown with light microscopy Photo_15.tif=Cultures shown in Photo_14, having subsequently developed into biflagellated cells with a rudimentary haptoneme Photo_16.tif=Shaved area of Lithothamnion sp. 2 rhodolith from Ewing Bank exposing brown patches of non-flagellated O. verrucosa Photo_17.tif=Cultures of non-flagellated cells isolated from within the rhodolith shown with SEM

Individual Files: