Abstract:

Seawater samples were collected and incubated at near-in-situ temperatures to investigate the biochemical and isotopic kinetics of aerobic methane oxidation. Samples were collected in the Gulf of Mexico in 2015 and US Atlantic Margin in 2014. A new technique was developed for the incubation and analysis of these samples, which can be found in the publication: Chan, E. W., J. D. Kessler, A. M. Shiller, D. J. Joung, and F. Colombo (2016). Aqueous Mesocosm Techniques Enabling the Real-Time Measurement of the Chemical and Isotopic Kinetics of Dissolved Methane and Carbon Dioxide, Environmental Science & Technology, 50(6), 3039-3046 doi:10.1021/acs.est.5b04304.

Suggested Citation:

John Kessler, Eric Chan. 2018. Chemical and Isotopic Kinetics of Dissolved Methane and Carbon Dioxide for samples collected in the northern Gulf of Mexico and Atlantic July 2014-April 2015. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7RR1WPX

Publications:

Shiller, A. M., Chan, E. W., Joung, D. J., Redmond, M. C., & Kessler, J. D. (2017). Light rare earth element depletion during Deepwater Horizon blowout methanotrophy. Scientific Reports, 7(1). doi:10.1038/s41598-017-11060-z

Purpose:

Microbial aerobic oxidation in seawater is a significant sink of dissolved methane. Here we investigate the chemical and isotopic kinetics of this process by incubating and analyzing seawater samples.

Data Parameters and Units:

Sample name, Date collected (MMDDYYYY), Latitude (decimal degrees), Longitude (decimal degrees), Depth (m), temperature (degrees C), Start/End time (days since initialization), salinity (per mille), dissolved oxygen (uM), phosphate (uM), silicate (uM), nitrite (uM), nitrate (uM), ammonia (uM), La (pM), Ce (pM), Pr (pM), Nd (pM), Sm (pM), Eu (pM), Mn (nM), Fe (nM), Cu (nM), Zn (nM), Mo (nM), dissolved CH4 (mM), Dissolved Oxygen (uM), Carbon cell counts (cells/liter), dissolved Carbon Dioxide (uM), abundance of 16s rRNA (percent), stable carbon isotopic composition of CH4 (del13C-CH4) (per mille), stable carbon isotopic composition of carbon dioxide (del13C-CO2) (per mille).

Methods:

A new technique was developed for the incubation and analysis of these samples, which can be found here. Chan, E. W., J. D. Kessler, A. M. Shiller, D. J. Joung, and F. Colombo (2016), Aqueous Mesocosm Techniques Enabling the Real-Time Measurement of the Chemical and Isotopic Kinetics of Dissolved Methane and Carbon Dioxide, Environmental Science & Technology, doi: 10.1021/acs.est.5b04304

Provenance and Historical References:

Dataset was made available on September 14 2017. The contents of the file HC-S5-CH4.csv were duplicated in the file HC-S5-CO2.csv. This duplication was identified and the file HC-S5-CO2.csv was updated on June 26 2018.

Individual Files: