Abstract:

Impacts of oil exposure on fertility potential of affected organisms can allow us to answer questions regarding serious reproductive impairment. Several reproductive hormones were used to establish exposure level impacts on targeted fish species. Enzyme linked immunoassays were performed on blood serum samples-concentrations of hormones and are reported as pg/ml of serum.

Suggested Citation:

Wetzel, Dana. 2016. Fertility Potential Assays, DeSoto Canyon, August 15-29, 2013. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7WM1BF1

Purpose:

Monitor levels of Inhibin A to determine impacts on fertility potential.

Data Parameters and Units:

Plate (ID), Assay (ID), Curve Fit Type [linear regression, R-squared], Well Positions (ID), Sample (ID), Raw Data, Curve Fit, Final Results, Sample (ID), Inhibin A Rep 1 to Rep 2 (pg/mL), Inhibin A Average (pg/mL), Inhibin A % CV coefficient variation of absorbance (%). The following is a list of stations where samples in the dataset were collected from (Station ID, Westbound Extent, Northbound Extent, Southbound Extent, Eastbound Extent): WB-SL10-40, -88.52829, 29.12272, 29.08907, -88.52213; WB-HE-265, -90.30623, 28.23532, 28.22594, -90.28127; WB-SL16-150, -90.00278, 28.3727, 28.3387, -89.3387; WB-SL4-40, -84.25941, 28.07729, 28.05337, -84.24252; WB-SL7-150, -86.4291, 29.29186, 29.27112, -86.40274; WB-SL14-60, -87.25733, 29.2971, 29.27837, -87.22894; "Inhibin A 2013 Sample Locations.csv": Fish Sample ID (unique identifier that corresponds to the Sample ID fields in the other dataset files), Station ID (station where sample was collected), Species (common name) [Red Snapper, Golden tilefish], Westbound Extent (western bounding extent of station (DD)), Northbound Extent (northern bounding extent of station (DD)), Southbound Extent (southern bounding extent of station (DD)), Eastbound Extent (eastern bounding extent of station (DD)), Center Point Lat (latitudinal center point of station bounding extent(DD)), Center Point Lon (longitudinal center point of station bounding extent (DD)).

Methods:

Sample Collection: Serum Collect 7.5 mL whole blood into a marbletop serum separator tube. Invert a few times and allow to clot on ice for 30 minutes. Spin at 2700 rpm for 15 minutes. Remove supernatant and place in a red top cryovial. Store in -20 freezer or in LN2. When samples are removed and thawed for the first time they are aliquotted out to prevent numerous freeze/thaw cycles Sample Processing: Samples are run according to manufacturer's protocol.

Instruments:

DS2 Automated ELISA instrument by Dynex Technologies Inhibin A human ELISA by Beckman-Coulter

Error Analysis:

Standard curves and no substrate blanks are run with each plate. High and low controls are included on Inhibin A plates Samples are run in duplicate Sample %CV's are calculated and outliers are removed such that %CV is less than 20%

Provenance and Historical References:

WB-PCB03-100, WB-SL7-150, WB-SL14-60, WB-SL14-100, WB-SL8-100, WB-SL9-80, WB-SL9-150, WB-SL10-40, WB-HE-265, WB-SL16-150, WB-SL12-100, WB-SL11-150, WB-SL5-200, WB-SL5-100, WB-SL4-40

Individual Files: