Abstract:

Vitellogenin is an egg-yolk precursor protein expressed in females of nearly all oviparous species and is commonly used to detect estrogenic effects in male fish. Vitellogenin antibodies were validated for each of the collected fish species and will be analyzed used appropriate antibodies. The vitelligenin assays-concentrations were reported as pg/ml of plasma.

Suggested Citation:

Wetzel, Dana. 2016. Plasma vitellogenin levels of collected fish species, DeSoto Canyon, August 15-29, 2012. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7542KM6

Purpose:

Assessment of vitellogenin levels in plasma to determine liklihood of endocrine disruption.

Data Parameters and Units:

Plate (ID), Assay (ID), Curve Fit Type [linear regression, R-squared], Well Positions (ID), Sample (ID), Raw Data, Curve Fit, Final Results, Sample (ID), Sex (M,F), Species (common name) [Black Line Tilefish, Blue Line Tilefish, Red Grouper, Red Snapper, Snowy Grouper, Tilefish, Yellow Edge Grouper], Vitellogenin Concentration Rep 1 to Rep 3 (ng/mL, pg/mL), Vitellogenin Concentration Average (pg/mL), Vitellogenin Concentration % CV coefficient variation of absorbance (%), Vitellogenin Absorbance Rep 1 to Rep 3 (AU), Vitellogenin Absorbance Average (AU). The following is a list of stations where samples in the dataset were collected from (Station ID, Westbound Extent, Northbound Extent, Southbound Extent, Eastbound Extent): WB-SL7-150, -86.42981, 29.29443, 29.27616, -86.39935; WB-SL8-100, -87.14303, 29.445, 29.41751, -87.12102; WB-SL9-80, -88.00592, 29.17805, 29.16829, -88.04336; WB-SL9-150, -88.00322, 29.1495, 29.14935, -87.5974; WB-SL10-40, -88.53312, 29.1116, 29.08347, -88.52524; WB-SL14-100, -87.3263, 29.24353, 29.22158, -87.29868; WB-SL14-60, -87.26249, 29.30115, 29.27621, -87.24361; WB-PCB03-100, -86.24402, 29.43523, 29.41358, -86.20798; WB-SL8-40, -87.16775, 29.5302, 29.51386, 87.13137; WB-16-150, -90.00237, 28.38246, 28.34908, -89.58652; The dataset file titled " Vitellogen 2012 Sample Locations" contains information about where each sample in the dataset was collected from and is organized by Plate ID. The following is a description of each field in the file: Fish Sample ID (unique identifier that corresponds to the Sample ID fields in the other dataset files), Station ID (station where sample was collected), Westbound Extent (western bounding extent of station (DD)), Northbound Extent (northern bounding extent of station (DD)), Southbound Extent (southern bounding extent of station (DD)), Eastbound Extent (eastern bounding extent of station (DD)), Center Point Lat (latitudinal center point of station bounding extent(DD)), Center Point Lon (longitudinal center point of station bounding extent (DD)).

Methods:

Sample Collection: plasma - Collect 4 mL whole blood into a green top lithium heparin tube containing aprotinin, - Invert the tube 5 times and allow to clot on ice for 30 minutes, - Spin at 2700 rpm for 15 minutes, - Remove supernatant and place in green top cryovial containing aprotinin; - Store in -20 freezer or in LN2. Sample Processing: Samples are run according to manufacturer's protocol.

Instruments:

DS2 Automated ELISA instrument by Dynex Technologies; Vitellogenin Rainbow Trout ELISA by Biosense Laboratories

Error Analysis:

Standard curves and no substrate blanks are run with each plate; Samples are run in triplicate; Sample %CV's are calculated and outliers are removed such that %CV is less than 20%.

Individual Files: