Abstract:

This dataset contains the relative abundances of intact polar membrane lipids of bacteria (specifically, bacteriohopanepolyol lipids) in northern Gulf of Mexico sediments. Samples used in the analysis were collected in August, 2013. Values reported are the mean of duplicate or triplicate measurements. All compounds are normalized to an internal standard. The sample collection dates and the number of replicates used to calculate the mean for each sample are listed in the supplemental information of the metadata record.

Suggested Citation:

Lincoln, Sara. 2016. Intact polar lipids in sediments impacted by the Deepwater Horizon oil spill. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7513W7B

Purpose:

The analysis focused on the bacteriohopanepolyol class of intact polar membrane lipids because they are taxonomically specific for certain bacterial groups that were likely involved in hydrocarbon degradation post-Deepwater Horizon spill.

Data Parameters and Units:

Relative abundance of intact polar lipids presented as a percent of total intact polar lipids measured (assuming a 1:1 response factor between each intact polar lipid and the internal standard pregnane diacetate), normalized to the peak area of a given mass of an internal standard used in analyses. When possible, response factors for individual lipids were determined using authentic standards. The following are the number of replicates used to calculate the mean for each sample: SL1040 - 3 replicates; DSH 08 - 2 replicates; DSH 10 - 2 replicates; PCB 06 - 3 replicates; The following are the sample dates for each site: SL1040 - 8/21/2013; DSH 08 - 8/16/2013; DSH 10 - 8/20/2013; PCB 06 - 8/16/2013;

Methods:

Liquid chromatography – mass spectrometry

Instruments:

Agilent liquid chromatograph coupled to an Agilent ion trap mass spectrometer

Individual Files: