Abstract:

Six cohesive sand patty sediments were collected on a West Florida Shelf beach in St. Petersburg Florida, January, 2013. These were analyzed for presence of PAH compounds and the composition compared to measurements from known Deepwater Horizon contaminated beach sediments. Known contaminated samples were collected from Santa Rosa Florida in July 2010 and January 2011, and from Gulf Shores Alabama in December 2012. The same samples were also analyzed for biological activity and the presence of Corexit dispersant (DOSS). This dataset includes concentration data for PAHs and DOSS in these samples. The biological activity in the water accommodated fraction of samples was measured through Microtox toxicity and Microscreen mutagenicity. The data provided for these analyses includes luminescence, and percent change and inhibition compared to controls. DOSS concentration analysis, microtox and microscreen assay data for control sand, loose beach sand collected in proximity to sand patty samples, is also provided in this dataset. Phenol controls were employed in Microtox analysis, and luminescence data is provided for controls.

Suggested Citation:

McDaniel, Lauren D, et al. 2015. West Florida Shelf beach sediment PAH and dioctyl sodium sulfosuccinate concentrations, microtox luminescence and microscreen mutagenicity data, 2010 to 2013. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N74M92J8

Publications:

McDaniel, L. D., Basso, J., Pulster, E., & Paul, J. H. (2015). Sand patties provide evidence for the presence of Deepwater Horizon oil on the beaches of the West Florida Shelf. Marine Pollution Bulletin. doi:10.1016/j.marpolbul.2015.06.032

Purpose:

To determine if Deepwater Horizon oil reached the beaches of the West Florida Shelf

Data Parameters and Units:

File: JBSediment_FinalDataGRIID-C_7-6-15.xlsx. Concentrations of polycyclic aromatic hydrocarbons (PAHs) from sediment samples (micrograms per gram dry weight) for 5 samples from St. Petersburg Florida, 1 from Sunset Beach Florida, 2 from Santa Rosa Florida collected in 2010 and 2011, 1 from Gulf Shores Alabama collected 2012 and 1 from Fort Pickens Florida collected in 2013; Latitude/Longitude = latitude and longitude where each sample was collected (decimal degrees) Collection Date = date that sample was collected (mm/dd/yyyy) Field ID= internal field id of sample MML ID = internal identifier for sample File: DOSS Analysis 10-2-14.xlsx. Concentrations of dioctyl sodium sulfosuccinate (DOSS) in nanograms per gram dry weight for five sediment samples collected from St. Petersburg Florida (Sunset Beach, sediment 1, sediment 2, sediment 3, sediment 4, sediment 5) , 1 sample collected from Fort Pickens Florida, and control sand File: Microtox_SBSediments_GRIIDC-C7-6-15.xlsx Worksheet: Combined sediment microtox Sample = sample name Date Sampled = date sample was collected Sampling Location = latitude and longitude where sample was collected (decimal degrees) Microtox Toxicity as Percentage Change over control = % inhibition of luminescence compared to control SD = standard deviation of Microtox toxicity as percentage change over control Worksheet: Microtox Sunset Beach Sediments Sample = sample ID Total sample Concentration (% of total) = concentration of sample used in analysis (samples were diluted with kit reagent) Gamma (luminescence in cd/m2) = luminescence in candela per square meter % Effect (Proportion = inhibition in comparison to negative control) = the inhibition of luminescence in the sample compared to negative control * 100 Ave. Effect = average % inhibition of comparison to negative controls File: Microscreen_SBSediments_GRIID-C7-8-15.xslx Sample Designation = sample name Date of Sampling = date sample was collected (mm/dd/yyyy) Latitude/Longitude = latitude and longitude where samples was collected (decimal degrees) Date Assayed = date Microscreen Assay performed on sample (mm/dd/yyyy) Microscreen % change over negative control = average percentage change in comparison to control for n = 4 samples SD = standard deviation of microscreen % change over negative control Columns H to J – combined microscreen results – in cases where samples were assayed twice the most recent value was used for analysis

Methods:

gas chromatography/mass spectrometry Microtox microbial toxicity assay, Microtox Model 500 (M500) temperature controlled photometer

Individual Files: