Abstract:

Experiments were performed in high pressure reactors at 150 bar and room temperature to determine the biodegradation of 3 mineral oil components (decane, hexadecane and tetracosane) by a hydrocarbon-degrading bacterial strain. The substrate degradation, bacterial growth and oxygen consumption were investigated.

Suggested Citation:

Schedler, Martina. 2015. Mineral Oil Components Degradation Curves and Bacterial Growth Curves in Experiments Performed in High Pressure Reactors. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7NK3C0V

Publications:

Valladares Juarez, A. G., Kadimesetty, H. S., Achatz, D. E., Schedler, M., & Muller, R. (2015). Online Monitoring of Crude Oil Biodegradation at Elevated Pressures. IEEE J. Sel. Top. Appl. Earth Observations Remote Sensing, 8(2), 872–878. doi:10.1109/jstars.2014.2347896

Lindo-Atichati, D., Paris, C. B., Le Hénaff, M., Schedler, M., Valladares Juárez, A. G., & Müller, R. (2016). Simulating the effects of droplet size, high-pressure biodegradation, and variable flow rate on the subsea evolution of deep plumes from the Macondo blowout. Deep Sea Research Part II: Topical Studies in Oceanography, 129, 301–310. doi:10.1016/j.dsr2.2014.01.011

Purpose:

Investigating the biodegradation of mineral oil components at high pressure and creating substrate degradation curves, oxygen consumption curves and growth curves.

Data Parameters and Units:

Below is a list of parameters for each dataset: Schedler_2014.xlsx - biodegradation of decane, hexadecane, tetracosane - time (hours), colonies (cells per milliliter), chemical concentration (millimoles), oxygen (percent); Schedler-high_pressure_biodegradation.xlsx - pressure (bar), incubation time (hour), standard deviation (cells per milliliter), CFU colony forming unit (cells per milliliter), chemical concentration (millimoles); Valladares-TotalDataGraphs-Database.xlsx - time (hours), carbon dioxide (millimoles per liter), oxygen (millimoles per liter), delta CFU (colony forming units;

Methods:

The growth curves were made by plate counting on LB-agar. The substrate degradation was measured by GC-MS (gas chromatograph mass-spectroscopy) in the liquid phase. The oxygen was measured online in the gas phase using a T1000 Hioxy (Ocean Optics) sensor.

Instruments:

High pressure reactors, GC-MS and T1000 Hioxy (Ocean Optics) sensor were used.

Individual Files: