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Seasurface Microlayer Project: relative abundance of surfactant-associated bacteria from 454 high-throughput sequencing

Authors: Kurata, N., Vella, K.,Hamilton, B., Shivji, M., Soloviev, A., Matt, S., Tartar, A., Pierrie, W.
Published On: Jun 21 2016 19:47 UTC
DOI: https://doi.org/10.7266/N7CV4FR6
UDI: R1.x134.073:0044
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Project Information

Funded By:
Gulf of Mexico Research Initiative

Funding Cycle:
RFP-I

Research Group:
Consortium for Advanced Research on Transport of Hydrocarbon in the Environment (CARTHE)

Point of Contact

Helga S. Huntley
University of Delaware / School of Marine Science and Policy
helgah@udel.edu

Data Collection Period
2010-07-10
to
2011-09-10
Theme keywords

surfactant, bacteria, 454 high-throughput sequencing, sea surface microlayer

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Abstract:

Microlayer samples were taken in 2010 and 2011 in the Straits of Florida approximately 8 miles east of Port Everglades Fort Lauderdale, on the Atlantic Coast of Broward County, Florida. This dataset contains the raw sequence reads from the 454 high-throughput sequencing.

Suggested Citation:

Kurata, N., Vella, K.,Hamilton, B., Shivji, M., Soloviev, A., Matt, S., Tartar, A., Pierrie, W.. 2016. Seasurface Microlayer Project: relative abundance of surfactant-associated bacteria from 454 high-throughput sequencing. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7CV4FR6

Publications:

Kurata, N., Vella, K., Hamilton, B., Shivji, M., Soloviev, A., Matt, S., … Perrie, W. (2016). Surfactant-associated bacteria in the near-surface layer of the ocean. Scientific Reports, 6, 19123. doi:10.1038/srep19123

Purpose:

The bacterial abundance was determined from 454 sequencing to help understand the difference between presence of surfactant-associated bacteria in and out of slicks the near-surface layer of the ocean and in subsurface waters.

Data Parameters and Units:

raw 16S rRNA sequence reads from the 454 high-throughput sequencing, collection date (YYYY-MM-DD), depth (m), latitude, longitude, wind direction (degrees), wind speed (m/s)

Instruments:

47 mm polycarbonate membrane filter