Abstract:

This dataset includes the results of a series of roller tank incubations with surface seawater from the Green Canyon oil reservoir, northern Gulf of Mexico, amended with either a natural oil slick (GCS-oil) or pristine oil. Incubations were sampled for bacterial enzyme activity and transparent exopolymer particles formation as well as bio-degradation of oil components.

Suggested Citation:

Kai Ziervogel. 2016. Alkane, transparent exopolymer particle and dissolved organic carbon concentrations and enzyme production rate data from surface seawater amended with oil. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7JS9NGV

Purpose:

The goal was to test whether bacterial activities of natural surface water communities facilitate the formation of oil-rich marine snow

Data Parameters and Units:

Alkanes.xlsx: column labeled Alkane Concentrations (ug/mL) is the alkane measured (C15-C40), each column reports alkane concentration (ug/mL) or percentage for each treatment [GCS-oil (natural slick oil, day 0); SW+GCS-oil (seawater and natural slick oil, day 4); SW+particles+GCS-oil (seawater, particles, and natural slick oil, day 4)] CN_new.xlsx: Carbon/Nitrogen ratios for replicates in treatments; [SW Day 0 (seawater, day 0); SW Day 2 (seawater, day 2); SW Day 4 (seawater, day 4); SW+NO Day 0 (seawater and natural oil, day 0); SW+NO Day 2 (seawater and natural oil, day 2); SW+NO Day 4 (seawater and natural oil, day 4); SW+SPM+NO Day 0 (seawater, particles, and natural oil, day 0); SW+SPM+NO (SSW) Day 2 (seawater, particles, and natural oil, day 2) -surrounding seawater sample; SW+SPM+NO (SSW) Day 4 (seawater, particles, and natural oil, day 4) -surrounding seawater sample; SW+SPM+NO (MS SL) Day 2 (seawater, particles, and natural oil, day 2) -macro-aggregate sample; SW+SPM+NO (MS SL) Day 4 (seawater, particles, and natural oil, day 4) -macro-aggregate sample; SW+SPM Day 0 (seawater and particles, day 0); SW+SPM (SSW) Day 2 (seawater and particles, day 2) -surrounding seawater sample; SW+SPM (SSW) Day 4 (seawater and particles, day 4) -surrounding seawater sample; SW+SPM (MS SL) Day 2 (seawater and particles, day 2) -macro-aggregate sample; SW+SPM (MS SL) Day 4 (seawater and particles, day 4) -macro-aggregate sample] correll.xlsx – enzyme hydrolysis rates, TEP concentrations, and oil fluorescence in treatments; Day (0, 2, 4), Treatment [(SW = seawater)(SW+oil = seawater and natural slick oil)], B glu cell-1 (β-glucosidase (amol cell−1 hr−1)), TEP (transparent exopolymer particles (μg GXeq tank−1)); oil fluores (oil fluorescence (relative fluorescence units)) DOC_new.xlsx: Dissolved organic Carbon concentrations for treatments; Dissolved Organic Carbon, (DOC, μg tank−1); treatment [SW Day 0 (seawater, day 0); SW Day 2 (seawater, day 2); SW Day 4 (seawater, day 4); SW+NO Day 0 (seawater and natural oil, day 0); SW+NO Day 2 (seawater and natural oil, day 2); SW+NO Day 4 (seawater and natural oil, day 4); SW+SPM+NO Day 0 (seawater, particles, and natural oil, day 0); SW+SPM+NO (SSW) Day 2 (seawater, particles, and natural oil, day 2) -surrounding seawater sample; SW+SPM+NO (SSW) Day 4 (seawater, particles, and natural oil, day 4) -surrounding seawater sample; SW+SPM Day 0 (seawater and particles, day 0); SW+SPM (SSW) Day 2 (seawater and particles, day 2) -surrounding seawater sample; SW+SPM (SSW) Day 4 (seawater and particles, day 4) -surrounding seawater sample] EEA_new.xlsx: enzymatic hydrolysis rates for replicates in treatments; Leu MCA cell-1 (L-leucine-MCA hydrochloride, a mol cell1 hr-1), Leu MCA tank-1 (L-leucine-MCA hydrochloride, tank-1), B glu cell-1 (β-glucosidase (amol cell−1 hr−1)); B glu tank-1 (β-glucosidase, tank-1); treatment [SW Day 0 (seawater, day 0); SW Day 2 (seawater, day 2); SW Day 4 (seawater, day 4); SW+NO Day 0 (seawater and natural oil, day 0); SW+NO Day 2 (seawater and natural oil, day 2); SW+NO Day 4 (seawater and natural oil, day 4); SW+SPM+NO Day 0 (seawater, particles, and natural oil, day 0); SW+SPM+NO (SSW) Day 2 (seawater, particles, and natural oil, day 2) -surrounding seawater sample; SW+SPM+NO (SSW) Day 4 (seawater, particles, and natural oil, day 4) -surrounding seawater sample; SW+SPM+NO (MS SL) Day 2 (seawater, particles, and natural oil, day 2) -macro-aggregate sample; SW+SPM+NO (MS SL) Day 4 (seawater, particles, and natural oil, day 4) -macro-aggregate sample; SW+SPM Day 0 (seawater and particles, day 0); SW+SPM (SSW) Day 2 (seawater and particles, day 2) -surrounding seawater sample; SW+SPM (SSW) Day 4 (seawater and particles, day 4) -surrounding seawater sample; SW+SPM (MS SL) Day 2 (seawater and particles, day 2) -macro-aggregate sample; SW+SPM (MS SL) Day 4 (seawater and particles, day 4) -macro-aggregate sample] POC_new.xlsx: Particulate organic Carbon concentrations for treatments; Particulate Organic Carbon (POC, μg tank−1); treatment [SW Day 0 (seawater, day 0); SW Day 2 (seawater, day 2); SW Day 4 (seawater, day 4); SW+NO Day 0 (seawater and natural oil, day 0); SW+NO Day 2 (seawater and natural oil, day 2); SW+NO Day 4 (seawater and natural oil, day 4); SW+SPM+NO Day 0 (seawater, particles, and natural oil, day 0); SW+SPM+NO (SSW) Day 2 (seawater, particles, and natural oil, day 2) -surrounding seawater sample; SW+SPM+NO (SSW) Day 4 (seawater, particles, and natural oil, day 4) -surrounding seawater sample; SW+SPM+NO (MS SL) Day 2 (seawater, particles, and natural oil, day 2) -macro-aggregate sample; SW+SPM+NO (MS SL) Day 4 (seawater, particles, and natural oil, day 4) -macro-aggregate sample; SW+SPM Day 0 (seawater and particles, day 0); SW+SPM (SSW) Day 2 (seawater and particles, day 2) -surrounding seawater sample; SW+SPM (SSW) Day 4 (seawater and particles, day 4) -surrounding seawater sample; SW+SPM (MS SL) Day 2 (seawater and particles, day 2) -macro-aggregate sample; SW+SPM (MS SL) Day 4 (seawater and particles, day 4) -macro-aggregate sample] TEP_new.xlsx: Transparent exopolymer particle concentrations for treatments; transparent exopolymer particles (TEP, μg GXeq tank−1); Treatment [SW Day 0 (seawater, day 0); SW Day 2 (seawater, day 2); SW Day 4 (seawater, day 4); SW+NO Day 0 (seawater and natural oil, day 0); SW+NO Day 2 (seawater and natural oil, day 2); SW+NO Day 4 (seawater and natural oil, day 4); SW+SPM+NO Day 0 (seawater, particles, and natural oil, day 0); SW+SPM+NO (SSW) Day 2 (seawater, particles, and natural oil, day 2) -surrounding seawater sample; SW+SPM+NO (SSW) Day 4 (seawater, particles, and natural oil, day 4) -surrounding seawater sample; SW+SPM+NO (MS SL) Day 2 (seawater, particles, and natural oil, day 2) -macro-aggregate sample; SW+SPM+NO (MS SL) Day 4 (seawater, particles, and natural oil, day 4) -macro-aggregate sample; SW+SPM Day 0 (seawater and particles, day 0); SW+SPM (SSW) Day 2 (seawater and particles, day 2) -surrounding seawater sample; SW+SPM (SSW) Day 4 (seawater and particles, day 4) -surrounding seawater sample; SW+SPM (MS SL) Day 2 (seawater and particles, day 2) -macro-aggregate sample; SW+SPM (MS SL) Day 4 (seawater and particles, day 4) -macro-aggregate sample] data all.xlsx: Particulate Organic Carbon (POC tank-1, μg tank−1); Particulate Organic Carbon (POC L-1, ); Dissolved Organic Carbon, (DOC tank-1, μg tank−1); Dissolved Organic Carbon (DOC L-1, ); transparent exopolymer particles (TEP tank-1, μg GXeq tank−1); transparent exopolymer particles (TEP L-1, ); Bacterial cells (Leu MCA L-1, ); Bacterial cells (Leu MCA tank-1, × 10−8 tank−1); Bacterial cells (Leu cell-1, ); β-glucosidase (B glu L-1, ); β-glucosidase (B glu tank-1, ); (Glu cell-1, ); SW Day 0 (seawater, day 0); SW Day 3 (seawater, day 3); SW+PO Day 3 (seawater and pristine oil, day 3)

Individual Files: