Abstract:

Cultured isolates of the genus Halomonas were tested for the ability to emulsify and degrade aromatic hydrocarbons as well as their ability to form oil aggregates. High-throughput DNA sequencing was also used to determine the ambient abundance of these culture strains during the Deepwater Horizon blowout. Pyrosequencing data were submitted to the European Nucleotide Archive Sequence Read Archive under the study accession number ERP002443. The 16S rRNA gene sequence of Halomonas strains GOS-2, GOS-3a and TGOS-10 were deposited with GenBank under accession numbers JQ246430, JQ246431 and JQ246432, respectively. This dataset reports disintegrations per minutes (dmp), hydrocarbon concentrations, and absorbance from experiments reported in the publication Gutirrez et al 2013, Role of Bacterial Exopolysaccharides (EPS) in the Fate of the Oil Released during the Deepwater Horizon Oil Spill, PLOS ONE, 8 (6):e67717 doi:10.1371/journal.pone.0067717.

Suggested Citation:

Andreas Teske. 2016. Oil solubilization and degradation by exopolysaccharide producing Halomonas strains. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7S46PZ1

Publications:

Arnosti, C., Ziervogel, K., Yang, T., & Teske, A. (2014). Oil-derived marine aggregates – hot spots of polysaccharide degradation by specialized bacterial communities. Deep Sea Research Part II: Topical Studies in Oceanography. doi:10.1016/j.dsr2.2014.12.008

Gutierrez, T., Berry, D., Yang, T., Mishamandani, S., McKay, L., Teske, A., & Aitken, M. D. (2013). Role of Bacterial Exopolysaccharides (EPS) in the Fate of the Oil Released during the Deepwater Horizon Oil Spill. PLoS ONE, 8(6), e67717. doi:10.1371/journal.pone.0067717

Gutierrez, T., Singleton, D. R., Berry, D., Yang, T., Aitken, M. D., & Teske, A. (2013). Hydrocarbon-degrading bacteria enriched by the Deepwater Horizon oil spill identified by cultivation and DNA-SIP. ISME J, 7(11), 2091–2104. doi:10.1038/ismej.2013.98

Purpose:

The purpose of these data was to determine the capacity of Halomonas EPS to influence the dissolution of aromatic hydrocarbons and enhance the bioavailability of these compounds and rate of biodegradation by the in situ sea surface microbial community in the Gulf of Mexico

Data Parameters and Units:

C14 Expt_300710.xlsx - dmp (disintegrations per minute) as 14-labelled-CO2 after 3, 6, 9, 10 days in 3 replicates for control and treatment [treatment- TG39 cells (from colony) in 5 mL ONR7a + C12 HEX + C14 HEX (20,000 dpm)]; file C14 Expt 301109.xlsx - dmp (disintegrations per minute) as 14-labelled-CO2 after 3, 7, 9 days, for 3 replicates in live and killed treatments with substrates (glucose, phenanthrene, anthracene, pyrene, fluoranthene, naphthalene, BaP, chrysene, phthalate, salicylate, p-cresol, o-cresol, decane); Data_110810.xslx - absorbance at 251 nm, concentration of phenanthrene (mg/L) after 0, 2, 5, 8, 10, 12 days for 3 replicates in control and treatments (polymer HE39 at 0.1 mg/mL, 0.2 mg/mL, 0.4 mg/mL); Exp_110810.jnb - testing polymer HE39 to increase degradation of phenanthrene by Gulf water samples - average phenanthrene concentration (mg/L), day (0, 5, 12, 17) HE39 concentration (0 mg/mL, 0.1 mg/mL, 0.2 mg/mL, 0.4 mg/mL); Exp_140710 .jnb - testing polymer HE-39 for ability to increase degradation of phenanthrene by strain TG408 - average phenanthrene concentration (mg/L), day (0, 1, 3, 5, 8, 12), HE39 concentration (0mg/mL, 0.1mg/mL, 0.2 mg/mL, 0.4 mg/mL); Guitterez et al Plos1 tabe 2.csv - Substrate; Growth (+, positive, –, negative, w, weakly positive, ND, not determined), Minteralization (+, positive, –, negative, w, weakly positive, ND, not determined), Guitterez et al Plos1.csv - Sample ID, Date Collected (MMM DD), Depth (m), Latitude, Longitude, Distance from Wellhead (miles, mi); PAH expt data_240808-2.xlsx - replicates from treatments (Poly39 in PBS concentration 0 mg/mL, 0.25 mg/ml, 0.5 mg/ml, 0.75 mg/ml, 1 mg/ml and Poly39 in ONR7a concentration 0 mg/ml, 0.1 mg/ml, 0.2 mg/ml, 0.4 mg/ml), hydrocarbon (phenanthrene, pyrene, biphenyl, fluorene) concentration (mg/L); PAH expt_240808.jnb - poly conc in PBS (0 mg/mL, 0.25 mg/ml, 0.5 mg/ml, 0.75 mg/ml, 1 mg/ml), poly conc in ONR7a (0 mg/ml, 0.1 mg/ml, 0.2 mg/ml, 0.4 mg/ml), average concentration of hydrocarbon (phenanthrene, pyrene, biphenyl, fluorene) (mg/L)

Error Analysis:

Pyrosequencing data were submitted to the European Nucleotide Archive Sequence Read Archive under the study accession number ERP002443 The 16S rRNA gene sequence of Halomonas strains GOS-2, GOS-3a and TGOS-10 were deposited with GenBank under accession numbers JQ246430, JQ246431 and JQ246432, respectively.

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