Abstract:

Hydrocarbon plume and non-plume deep water samples were collected from six sites near the Deepwater Horizon oil spill site May-June 2010. We measured exo-acting enzyme activity to determine enzymatic hydrolysis of carbohydrates in samples collected inside and outside the plume. Two 15 mL deepwater plume and non-plume samples were incubated with a single fluorescently labeled laminarin, xylan, and chondroitin substrate at a concentration of 3.5 uM monomer equivalent. Samples collected after 0, 1, 3, 7, and 14 days were analyzed by gel-permeation chromatography with fluorescence detection to determine hydrolysis rates. Plume and non-plume samples were filtered through a isopore filter and examined with epiflourescence microscopy to count microbial cells. This dataset reports enzymatic activities for beta glucosidase and leucine aminopeptidase, polysaccharide hydrolysis and bacterial cell counts for plume and non-plume water samples.

Suggested Citation:

Kai Ziervogel. 2016. Dataset for: Enhanced protein and carbohydrate hydrolyses in plume-associated deepwaters initially sampled during the early stages of the Deepwater Horizon oil spill. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7T72FGP

Publications:

Gutierrez, T., Berry, D., Yang, T., Mishamandani, S., McKay, L., Teske, A., & Aitken, M. D. (2013). Role of Bacterial Exopolysaccharides (EPS) in the Fate of the Oil Released during the Deepwater Horizon Oil Spill. PLoS ONE, 8(6), e67717. doi:10.1371/journal.pone.0067717

Gutierrez, T., Singleton, D. R., Berry, D., Yang, T., Aitken, M. D., & Teske, A. (2013). Hydrocarbon-degrading bacteria enriched by the Deepwater Horizon oil spill identified by cultivation and DNA-SIP. ISME J, 7(11), 2091–2104. doi:10.1038/ismej.2013.98

McKay, L. J., Gutierrez, T., & Teske, A. P. (2013). Development of a group-specific 16S rRNA-targeted probe set for the identification of Marinobacter by fluorescence in situ hybridization. Deep Sea Research Part II: Topical Studies in Oceanography. doi:10.1016/j.dsr2.2013.10.009

Ziervogel, K., & Arnosti, C. (2013). Enhanced protein and carbohydrate hydrolyses in plume-associated deepwaters initially sampled during the early stages of the Deepwater Horizon oil spill. Deep Sea Research Part II: Topical Studies in Oceanography. doi:10.1016/j.dsr2.2013.09.003

Purpose:

Sampling was part of our rapid response efforts to collect as many water samples as possible to determine bacterial community activities in waters affected by the spill.

Data Parameters and Units:

read me: Sample ID, Station ID, Sampling Date (MM/DD/YYYY), Latitude (°N), Longitude (°W), Sampling Depth (m), Sample Description/oil contamination, Distance to wellhead (km). EA b-glu: EA b-glu (Enzyme activities beta glucosidase), Two point fluorescence measurement with MUF-β-D-glucopyranoside (final substrate concentration in 2 mL of seawater: 1000 uM), Tube, t0 fl (fluorescence reading at the beginning of the incubation), Start (start time, HH:MM:SS); End (end time, HH:MM:SS), t1 fl (fluorescence reading at the end of the incubation), time elapsed (HH:MM:SS), fl elapsed (fluorescence reading at the end of incubation), fl elapsed - control (fluorescence reading at the end of incubation minus the control fluorescence average), hβ-glucosidase activity (nmol L-1 h-1), plume (deep water samples that had a distinct CDOM signal, detected with CDOM sensor/CTD), non-plume (deep water samples that had no CDOM signal, detected with CDOM sensor/CTD), control (deep water sample that was autoclaved). EA peptidase: EA peptidase (Enzyme activities leucine aminopeptidase), Two point fluorescence measurement with L-leucine-4-methylcoumarinyl-7-amide hydrochloride (final substrate concentration in 2 mL of seawater: 1200 uM), Tube, t0 fl (fluorescence reading at the beginning of the incubation), Start (start time, HH:MM:SS); End (end time, HH:MM:SS), t1 fl (fluorescence reading at the end of the incubation), time elapsed (HH:MM:SS), fl elapsed (fluorescence reading at the end of incubation), fl elapsed - control (fluorescence reading at the end of incubation minus the control fluorescence average), Peptidase activity (nmol L-1 h-1), plume (deep water samples that had a distinct CDOM signal, detected with CDOM sensor/CTD), non-plume (deep water samples that had no CDOM signal, detected with CDOM sensor/CTD), control (deep water sample that was autoclaved). polysaccharide rates: polysaccharide rates (Hydrolysis rates of three fluorescently-labeled polysaccharides: Laminarin, Xylan, Chondroitin sulfate), laminarin hydrolysis rate (nmol L-1 h-1), xylan hydrolysis (nmol L-1 h-1), chondroitin hydrolysis (nmol L-1 h-1), incubation time (hours), plume (deep water samples that had a distinct CDOM signal, detected with CDOM sensor/CTD), non-plume (deep water samples that had no CDOM signal, detected with CDOM sensor/CTD), control (deep water sample that was autoclaved). bacterial cell counts: bacterial cell counts (results from direct counts of DAPI stained cells; two filters per sample), plume (deep water samples that had a distinct CDOM signal, detected with CDOM sensor/CTD), non-plume (deep water samples that had no CDOM signal, detected with CDOM sensor/CTD), control (deep water sample that was autoclaved), bacterial abundance (cells mL-1), filter area (um2), area of pic (um2), volume of sample (ml), M (filter area um2/pic area um2)

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