Abstract:

DNA-based stable-isotope probing with uniformly (13)C-labelled hydrocarbons followed by DNA sequencing was used to identify oil degrading bacteria present in surface and oil plume water associated with the Deepwater Horizon blowout. Further, qPCR specific to the groups identified as being involved in oil degradation via DNA-SIP was performed to show these groups respond to oil addition with increased abundance. This dataset includes 16S rRNA gene abundances acquired via qPCR. Data were generated through laboratory incubations of surface water collected near 28° 4.175N 88°22.335 W on May 5th 2010 and within plume water collected on May 31st 2010 at 28° 41.686 N 88° 26.081 W. The dataset listed under this UDI: R1.x132.135:0001 - is exclusive to Hydrocarbon-degrading bacterial DNA sequence and abundance.

Suggested Citation:

Andreas Teske. 2016. Bacterial 16s rRNA gene abundance based on quantitative PCR data from incubations with hydrocarbons. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7ZP445D

Publications:

Gutierrez, T., Singleton, D. R., Berry, D., Yang, T., Aitken, M. D., & Teske, A. (2013). Hydrocarbon-degrading bacteria enriched by the Deepwater Horizon oil spill identified by cultivation and DNA-SIP. ISME J, 7(11), 2091–2104. doi:10.1038/ismej.2013.98

McKay, L. J., Gutierrez, T., & Teske, A. P. (2013). Development of a group-specific 16S rRNA-targeted probe set for the identification of Marinobacter by fluorescence in situ hybridization. Deep Sea Research Part II: Topical Studies in Oceanography. doi:10.1016/j.dsr2.2013.10.009

Purpose:

The purpose of this work was to determine the identity of bacteria involved in oil-degradation in the wake of the Deepwater Horizon blowout and confirm their ability to degrade oil.

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